Determining metabolic mechanism linking phospholipids and docosahexaenoic acid through phosphatidylcholine synthesis by phosphocholine cytidylyltransferase (CCT) overexpression in Schizochytrium sp.

IF 4.3 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbial Cell Factories Pub Date : 2025-04-04 DOI:10.1186/s12934-025-02703-2

Xiaowen Cui, Tingting Chen, Yizhen Meng, Xueshan Pan, Ruizhe Wu, Yinghua Lu, Chuanyi Yao, Xihuang Lin, Xueping Ling

{"title":"Determining metabolic mechanism linking phospholipids and docosahexaenoic acid through phosphatidylcholine synthesis by phosphocholine cytidylyltransferase (CCT) overexpression in Schizochytrium sp.","authors":"Xiaowen Cui, Tingting Chen, Yizhen Meng, Xueshan Pan, Ruizhe Wu, Yinghua Lu, Chuanyi Yao, Xihuang Lin, Xueping Ling","doi":"10.1186/s12934-025-02703-2","DOIUrl":null,"url":null,"abstract":"<p><p>The polyunsaturated fatty acid (PUFA) metabolism of Schizochytrium, an excellent oil-producing microorganism, is closely related to phosphatidylcholine (PC) synthesis, which favors the migration and accumulation of docosahexaenoic acid (DHA). Phosphocholine cytidylyltransferase (CCT), a key enzyme involved in PC synthesis, profoundly impacts lipid metabolism in plants; however, few studies have focused on CCT in microorganisms. We investigated the effects of CCT overexpression on lipid metabolism in Schizochytrium sp. CCT overexpression slightly inhibited cell growth, but significantly promoted total lipid synthesis. Compared to the wild-type strain, PUFA content and DHA production in the CCT-overexpressing strain (SR21-CCT) increased by about 49% and 46%, respectively. Analysis of phospholipids and quantitative real-time PCR revealed that CCT overexpression enhanced phospholipid synthesis, especially by strengthening glycerophosphorylcholine acylation and de novo PC synthesis pathways, which promote DHA esterification to PC and DHA accumulation in triacylglycerols. This study helps decipher the mechanism correlating phospholipid metabolism and DHA production.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"81"},"PeriodicalIF":4.3000,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969949/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial Cell Factories","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s12934-025-02703-2","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The polyunsaturated fatty acid (PUFA) metabolism of Schizochytrium, an excellent oil-producing microorganism, is closely related to phosphatidylcholine (PC) synthesis, which favors the migration and accumulation of docosahexaenoic acid (DHA). Phosphocholine cytidylyltransferase (CCT), a key enzyme involved in PC synthesis, profoundly impacts lipid metabolism in plants; however, few studies have focused on CCT in microorganisms. We investigated the effects of CCT overexpression on lipid metabolism in Schizochytrium sp. CCT overexpression slightly inhibited cell growth, but significantly promoted total lipid synthesis. Compared to the wild-type strain, PUFA content and DHA production in the CCT-overexpressing strain (SR21-CCT) increased by about 49% and 46%, respectively. Analysis of phospholipids and quantitative real-time PCR revealed that CCT overexpression enhanced phospholipid synthesis, especially by strengthening glycerophosphorylcholine acylation and de novo PC synthesis pathways, which promote DHA esterification to PC and DHA accumulation in triacylglycerols. This study helps decipher the mechanism correlating phospholipid metabolism and DHA production.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Microbial Cell Factories 工程技术-生物工程与应用微生物

CiteScore

9.30

自引率

4.70%

发文量

235

审稿时长

2.3 months

期刊介绍： Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology. The journal is divided into the following editorial sections: -Metabolic engineering -Synthetic biology -Whole-cell biocatalysis -Microbial regulations -Recombinant protein production/bioprocessing -Production of natural compounds -Systems biology of cell factories -Microbial production processes -Cell-free systems