In Vitro Lethal Effects of Benzyl Benzoate Concentrations of 25%, 10%, and 5% on Demodex folliculorum Mites: An Experimental Study With a Control Group

IF 2.3 4区医学 Q2 DERMATOLOGY

Journal of Cosmetic Dermatology Pub Date : 2025-04-07 DOI:10.1111/jocd.70175

İlkay Can, Murat Durdu

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Abstract

Background

Demodex mites have been implicated in the pathogenesis of many dermatologic diseases, especially rosacea. Although many case reports have been published about the treatment of skin diseases caused by Demodex folliculorum mite, no relevant treatment algorithm has been developed so far. In this context, we investigated the lethal effects of different doses of benzyl benzoate on this mite.

Objective

To compare the lethal effects of 3 different doses of benzyl benzoate on Demodex folliculorum mites with those of a control group consisting of immersion oil.

Materials and Methods

Waste samples collected from rosacea patients were included in the study. Four different groups of 20 mites were formed. Each group was exposed to 5%, 10%, and 25% concentrations of benzyl benzoate, respectively. Immersion oil was used as a negative control group. The movements of the mites were monitored with a digital microscope. The time to death of the mites was recorded.

Results

The mean time to death of mites was 26 ± 2,9, 120 ± 7.6, and 168 ± 15 min after application of benzyl benzoate at concentrations of 25%, 10%, and 5%, respectively. The mean time to death of mites in immersion oil applied as a negative control group was 192 ± 6.4 min. Among the study groups, only the 25% benzyl benzoate group had a statistically significantly shorter mean time to death compared to the control group (p = 0.03).

Conclusion

While low concentrations of benzyl benzoate were ineffective in the treatment of demodicosis, benzyl benzoate at high concentrations, e.g., 25%, was found to be effective in its treatment.

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25%、10%和5%苯甲酸苄酯浓度对毛囊蠕形螨体外致死效果的实验研究

蠕形螨与许多皮肤病的发病机制有关，尤其是酒渣鼻。虽然关于毛囊蠕形螨引起的皮肤病的治疗已经发表了许多病例报告，但目前还没有开发出相关的治疗算法。在这种情况下，我们研究了不同剂量的苯甲酸苄酯对这种螨的致死效果。目的比较3种不同剂量苯甲酸苄酯对毛囊蠕形螨的致死效果与浸泡油对照。材料与方法采用酒渣鼻患者的粪便标本进行研究。形成了四组不同的螨，每组20只。各组分别暴露于5%、10%和25%浓度的苯甲酸苄酯。以浸油为阴性对照组。用数码显微镜监测螨虫的运动。记录了螨虫的死亡时间。结果25%、10%、5%浓度苯甲酸苄酯对螨虫的平均死亡时间分别为26±2、9、120±7.6、168±15 min；阴性对照组浸油对螨的平均死亡时间为192±6.4 min。在各研究组中，只有25%苯甲酸苄酯组与对照组相比，平均死亡时间有统计学意义上的缩短（p = 0.03）。结论低浓度苯甲酸酯对蠕虫病治疗无效，高浓度苯甲酸酯（如25%）对蠕虫病治疗有效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Cosmetic Dermatology DERMATOLOGY-

CiteScore

4.30

自引率

13.00%

发文量

818

审稿时长

>12 weeks

期刊介绍： The Journal of Cosmetic Dermatology publishes high quality, peer-reviewed articles on all aspects of cosmetic dermatology with the aim to foster the highest standards of patient care in cosmetic dermatology. Published quarterly, the Journal of Cosmetic Dermatology facilitates continuing professional development and provides a forum for the exchange of scientific research and innovative techniques. The scope of coverage includes, but will not be limited to: healthy skin; skin maintenance; ageing skin; photodamage and photoprotection; rejuvenation; biochemistry, endocrinology and neuroimmunology of healthy skin; imaging; skin measurement; quality of life; skin types; sensitive skin; rosacea and acne; sebum; sweat; fat; phlebology; hair conservation, restoration and removal; nails and nail surgery; pigment; psychological and medicolegal issues; retinoids; cosmetic chemistry; dermopharmacy; cosmeceuticals; toiletries; striae; cellulite; cosmetic dermatological surgery; blepharoplasty; liposuction; surgical complications; botulinum; fillers, peels and dermabrasion; local and tumescent anaesthesia; electrosurgery; lasers, including laser physics, laser research and safety, vascular lasers, pigment lasers, hair removal lasers, tattoo removal lasers, resurfacing lasers, dermal remodelling lasers and laser complications.