Comparative transcriptome analysis of head kidney and gill with infection of spring viremia of carp virus in common carp (Cyprinus carpio)

Abstract

Spring viremia of carp virus (SVCV) is the causative agent of SVC, which could cause serious economic losses in fish aquaculture, especially in common carp. To date, with high-throughput sequencing, there were studies focusing on molecular mechanism of SVCV infection at tissue or cell level of cyprinid fish. Nevertheless, no data was available on comparative transcriptome analysis of SVCV infection between mucosal and systemic lymphoid tissues. Here, transcriptome analysis of common carp head kidney and gill with SVCV was performed. It was shown that head kidney was more sensitive to SVCV than gill, with many more DEGs (2741 vs. 565). Moreover, compared with each other, DEGs of head kidney showed significant enrichment of signal transduction, especially for MAPK, TGF-β, FoxO and Wnt pathways, and metabolic response was more prominent in gill, although DEGs in these two tissues showed similar enrichment of KEGG signal pathways, including PRR pathways, cytokine-cytokine receptor interaction and necroptosis. Furthermore, among 380 common DEGs, CCL19 and IFI44L as key immune related genes were analyzed here. These results demonstrated tissue-specific influences caused by SVCV in vivo and provided new insights to better understand pathogenic and immunologic mechanism of SVCV, which are expected to be useful for development of control strategies to SVCV infection.