Quenching mechanism of dimethylaminocyanobiphenyl aggregates by fluorescein in CTAB: An interplay between FRET and electrostatic interactions

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-26 DOI:10.1016/j.saa.2025.126117

Arulmozhi Puhazhendhi, Pavan Kumar Mandali, Soumya Sivalingam

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Abstract

Fӧrster resonance energy transfer (FRET) is a widely used tool to study micro heterogeneous media. Herein, quenching mechanism of dimethylaminocyanobiphenyl aggregates in CTAB (DMACBA-S; donor) by fluorescein disodium salt (FLU; acceptor) was analyzed using steady-state and time-resolved fluorescence measurements. Even though, the quenching behavior resembled the characteristic FRET phenomenon, unusual spectral shifts in the emission maxima of DMACBA-S and FLU were observed. In-depth analysis of this uncommon spectral shifts and fluorescence decay curves of DMACBA-S in the presence and absence of FLU revealed that the quenching could be due to a combination of FRET and electrostatic interaction between the acceptor and the CTAB micelles which eventually resulted in the rupture of the micelle followed by the formation of weakly fluorescent DMACB aggregates in water (DMACBA-R). By varying the concentration of CTAB, a variation in the contribution from electrostatic interaction towards the quenching mechanism was observed. The study highlights the less known interaction of acceptor with the microheterogenous medium in FRET studies. The present results manifest the need for a deeper analysis of the experimental quenching data to avoid erroneous conclusions.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.