Human lymphocytes chromosome structure in the presence of magnesium ions

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-03-27 DOI:10.1016/j.tice.2025.102895

Astari Dwiranti , Kinanti Ayang Putri , Davita Adryanti Felicia Sampe , Shadira Anindieta Irdianto , Raden Hannie Dewi Hadyani Kartapradja , Anom Bowolaksono , Andi Salamah , Abinawanto

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引用次数: 0

Abstract

The magnesium ion is one of the divalent cations that have an important role in chromosome condensation. Research about the role of Mg^2 + has been conducted on the chromosomes from various types of cells, especially human cancer cell lines. Nonetheless, no research has been conducted on normal human cells, such as blood cells and lymphocytes. Therefore, a study to evaluate the effect of Mg²⁺ on chromosome structure in normal cells is required. This study aims to assess the effects of Mg²⁺ on chromosome structure in lymphocytes using GTL-banding and karyotyping. Metaphase chromosomes were obtained by culturing blood cells, followed by banding using Giemsa and Leishman stain. Mg²⁺ role was assessed using XBE5 solution (5 mM Mg²⁺) as control, XBE solution (0 mM Mg²⁺), and 1 mM EDTA solution as a cation chelator. The chromosome value from each treatment was evaluated using Quality Assessment from the Association for Clinical Cytogenetics according to the International System for Human Cytogenomics Nomenclature (ISCN). The result shows that the control chromosome had a compact structure with a clear banding pattern and bold band intensity, while those treated with XBE and EDTA had a less compact and fibrous structure with an unclear banding pattern and thin band intensity. In addition, the average chromosome score±SD of the XBE (4.389 ± 0.607) and EDTA-treated (4.611 ± 0.607) chromosomes were higher than those of the control (4.222 ± 0.427). These data indicated that Mg²⁺ is essential in maintaining the structure of the human lymphocyte chromosome.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.