A novel immobilization enzymatic system of nanocellulose-based β-glucosidase in O-glycoside bioconversion of phytochemicals

IF 4.9 2区工程技术 Q1 MATERIALS SCIENCE, PAPER & WOOD

Cellulose Pub Date : 2025-03-10 DOI:10.1007/s10570-025-06443-9

Jihong Cui, Lina Fu, Quan Zhou, Jiaqi Zhang, Xinlin Zhang, Jie Yang, Yujie Fu

引用次数: 0

Abstract

The rarely reported nanocellulose micropheres (NCMs) are promising carriers for enzyme immobilization. Herein, the present study successfully prepared an enzyme immobilization system based on modified nanocellulose microspheres with porous structure. The electrostatic attraction and covalent bonding between polydopamine/polyethyleneimine (PDA/PEI) co-modified nanocellulose microspheres (NCMs) and -COOH/-NH₂ residues in the enzyme achieved the immobilization of β-glucosidase (PDA/PEI@NCMs@BGL). PDA/PEI@NCMs@BGL exhibited excellent stability and reusability with 91.41% residual enzyme activity after 5 cycles and converted at least 90.47% of geniposide, polydatin, and genistin at 4 h. This study may lay a foundation for a cost-effective and environmentally friendly immobilized enzyme system based on modified nanocellulose for the bioconversion of phytochemicals.

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来源期刊

Cellulose 工程技术-材料科学：纺织

CiteScore

10.10

自引率

10.50%

发文量

580

审稿时长

3-8 weeks

期刊介绍： Cellulose is an international journal devoted to the dissemination of research and scientific and technological progress in the field of cellulose and related naturally occurring polymers. The journal is concerned with the pure and applied science of cellulose and related materials, and also with the development of relevant new technologies. This includes the chemistry, biochemistry, physics and materials science of cellulose and its sources, including wood and other biomass resources, and their derivatives. Coverage extends to the conversion of these polymers and resources into manufactured goods, such as pulp, paper, textiles, and manufactured as well natural fibers, and to the chemistry of materials used in their processing. Cellulose publishes review articles, research papers, and technical notes.