Gas1-high quiescent neural stem cells are multipotent and produce oligodendrocytes during aging and after demyelinating injury.

IF 9.8 1区生物学 Q1 Agricultural and Biological Sciences

PLoS Biology Pub Date : 2025-04-03 DOI:10.1371/journal.pbio.3003100

Chaoqiong Ding, Zhenzhong Pan, Xiang Yan, Ran Zhou, Huifang Li, Lu Chen, Yuan Wang, Yan Zhang

{"title":"Gas1-high quiescent neural stem cells are multipotent and produce oligodendrocytes during aging and after demyelinating injury.","authors":"Chaoqiong Ding, Zhenzhong Pan, Xiang Yan, Ran Zhou, Huifang Li, Lu Chen, Yuan Wang, Yan Zhang","doi":"10.1371/journal.pbio.3003100","DOIUrl":null,"url":null,"abstract":"<p><p>Quiescent neural stem cells (qNSCs) in the adult mouse subventricular zone (SVZ) normally have limited capacity to generate glia. Gliogenic domains are present in both dorsal and ventral SVZ, with the ventral region featuring a subpopulation of Gli1+ qNSCs. In dorsal SVZ, however, the molecular identity and developmental origin of oligodendrogenic qNSCs remains elusive. Here, through single-cell analysis and lineage tracing, we identify an undefined subpopulation of Gas1high qNSCs in dorsal SVZ, distinct from Gli1+ qNSCs. These cells originate from embryonic Gas1high dorsal radial glia, and persist into the aged SVZ. Remarkably, they are multipotent and more gliogenic than Gas1low/- qNSCs, continuously generating oligodendrocytes in the adult and aged brain, and can be mobilized for myelin repair upon demyelination. Together, our study uncovers a subpopulation of dorsally derived, multipotent long-term qNSCs in the adult and aged SVZ with enhanced gliogenic potential, shedding light on the heterogeneity and plasticity of NSCs in normal, aging, and disease conditions.</p>","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":"23 4","pages":"e3003100"},"PeriodicalIF":9.8000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PLoS Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1371/journal.pbio.3003100","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 0

Abstract

Quiescent neural stem cells (qNSCs) in the adult mouse subventricular zone (SVZ) normally have limited capacity to generate glia. Gliogenic domains are present in both dorsal and ventral SVZ, with the ventral region featuring a subpopulation of Gli1+ qNSCs. In dorsal SVZ, however, the molecular identity and developmental origin of oligodendrogenic qNSCs remains elusive. Here, through single-cell analysis and lineage tracing, we identify an undefined subpopulation of Gas1high qNSCs in dorsal SVZ, distinct from Gli1+ qNSCs. These cells originate from embryonic Gas1high dorsal radial glia, and persist into the aged SVZ. Remarkably, they are multipotent and more gliogenic than Gas1low/- qNSCs, continuously generating oligodendrocytes in the adult and aged brain, and can be mobilized for myelin repair upon demyelination. Together, our study uncovers a subpopulation of dorsally derived, multipotent long-term qNSCs in the adult and aged SVZ with enhanced gliogenic potential, shedding light on the heterogeneity and plasticity of NSCs in normal, aging, and disease conditions.

查看原文本刊更多论文

Gas1高度静止的神经干细胞具有多能性，可在衰老过程中和脱髓鞘损伤后产生少突胶质细胞。

成年小鼠心室下区（SVZ）的静止神经干细胞（qNSCs）通常产生胶质细胞的能力有限。胶质原结构域存在于SVZ的背侧和腹侧，腹侧区域具有Gli1+ qNSCs亚群。然而，在背侧SVZ中，寡突发生qNSCs的分子特征和发育起源仍然难以捉摸。在这里，通过单细胞分析和谱系追踪，我们在SVZ背部发现了一个未定义的gas1高qNSCs亚群，不同于Gli1+ qNSCs。这些细胞起源于胚胎时期的gas1高背径向胶质细胞，并持续存在到老年SVZ。值得注意的是，它们比Gas1low/- qNSCs具有多能性和更强的胶质源性，在成人和老年大脑中不断产生少突胶质细胞，并可在脱髓鞘时动员髓鞘修复。总之，我们的研究揭示了成人和老年SVZ中背源的多能长期qNSCs亚群，它们具有增强的胶质细胞生成潜力，揭示了NSCs在正常、衰老和疾病条件下的异质性和可塑性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

PLoS Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOLOGY

CiteScore

15.40

自引率

2.00%

发文量

359

审稿时长

3-8 weeks

期刊介绍： PLOS Biology is the flagship journal of the Public Library of Science (PLOS) and focuses on publishing groundbreaking and relevant research in all areas of biological science. The journal features works at various scales, ranging from molecules to ecosystems, and also encourages interdisciplinary studies. PLOS Biology publishes articles that demonstrate exceptional significance, originality, and relevance, with a high standard of scientific rigor in methodology, reporting, and conclusions. The journal aims to advance science and serve the research community by transforming research communication to align with the research process. It offers evolving article types and policies that empower authors to share the complete story behind their scientific findings with a diverse global audience of researchers, educators, policymakers, patient advocacy groups, and the general public. PLOS Biology, along with other PLOS journals, is widely indexed by major services such as Crossref, Dimensions, DOAJ, Google Scholar, PubMed, PubMed Central, Scopus, and Web of Science. Additionally, PLOS Biology is indexed by various other services including AGRICOLA, Biological Abstracts, BIOSYS Previews, CABI CAB Abstracts, CABI Global Health, CAPES, CAS, CNKI, Embase, Journal Guide, MEDLINE, and Zoological Record, ensuring that the research content is easily accessible and discoverable by a wide range of audiences.