Protumor effect of CXCL10/CXCR3 axis in canine mammary gland tumor.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Soyeon Cho, Na-Yon Kim, Yeseul Yang, Jae-Ha Jung, Dansong Seo, Soyun Jin, Sung-Hyun Hwang, Yongbaek Kim
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引用次数: 0

Abstract

Importance: Chemokines and their receptors play integral roles in carcinogenesis. CXC motif chemokine receptor 3 (CXCR3) in T cells mediates anti-tumor effects, whereas CXCR3 in malignant cells promotes proliferation and metastasis. Although the role of CXCR3 has been well-documented in human cancers, including breast cancer, its function in canine tumors remains largely unexplored.

Objective: This study aimed to investigate the effects of CXCR3 and its ligand interaction in canine mammary gland tumor (cMGT) cells.

Methods: CXCR3 expression in two cMGT cell lines, CIPp and CIPm, was evaluated using real-time quantitative polymerase chain reaction, western blotting, and flow cytometry. CXC motif chemokine ligand 10 (CXCL10)-induced changes in CXCR3 protein expression in cMGT cells were assessed using membrane fractionation assays. Cell proliferation and migration in response to CXCL10 treatment were analyzed using Cell Counting Kit-8 and wound-healing assays, respectively. Additionally, the downstream molecular mechanisms of the CXCL10/CXCR3 axis were examined.

Results: CXCR3 expression was significantly higher in CIPm than in CIPp cells. In both the cMGT cell lines, CXCL10 treatment reduced CXCR3 expression on the cell membrane in a dose- and time-dependent manner. The CXCR10/CXCR3 axis promoted cell proliferation and migration in cMGT cells. CXCL10/CXCR3 interaction upregulated the phosphorylation of AKT1 and ERK.

Conclusions and relevance: This study demonstrates that the CXCL10/CXCR3 axis may contribute to the pathogenesis of cMGTs by promoting tumor cell proliferation and migration. CXCR3 signaling represents a potential therapeutic target for cMGTs.

重要意义趋化因子及其受体在致癌过程中发挥着不可或缺的作用。T细胞中的CXC基团趋化因子受体3(CXCR3)具有抗肿瘤作用,而恶性细胞中的CXCR3则能促进增殖和转移。虽然 CXCR3 在人类癌症(包括乳腺癌)中的作用已得到充分证实,但其在犬肿瘤中的功能在很大程度上仍未得到探索:本研究旨在探讨 CXCR3 及其配体相互作用在犬乳腺肿瘤(cMGT)细胞中的影响:方法:使用实时定量聚合酶链反应、Western 印迹和流式细胞术评估了 CXCR3 在 CIPp 和 CIPm 两种 cMGT 细胞系中的表达。利用膜分离实验评估了 CXC motif 趋化因子配体 10(CXCL10)诱导的 cMGT 细胞中 CXCR3 蛋白表达的变化。细胞计数试剂盒 8 和伤口愈合试验分别分析了细胞增殖和迁移对 CXCL10 处理的反应。此外,还研究了 CXCL10/CXCR3 轴的下游分子机制:结果:CXCR3在CIPm细胞中的表达明显高于CIPp细胞。在两种 cMGT 细胞系中,CXCL10 处理都会以剂量和时间依赖的方式降低细胞膜上的 CXCR3 表达。CXCR10/CXCR3 轴促进了 cMGT 细胞的增殖和迁移。CXCL10/CXCR3相互作用上调了AKT1和ERK的磷酸化:本研究表明,CXCL10/CXCR3 轴可能通过促进肿瘤细胞的增殖和迁移而导致 cMGTs 的发病。CXCR3 信号转导是 cMGTs 的潜在治疗靶点。
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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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