Immortalized porcine mesangial cell line competent for the cultivation of Japanese encephalitis virus.

IF 1.5 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Veterinary Science Pub Date : 2025-03-01 DOI:10.4142/jvs.24222

Shucheng Zong, Xinze Liu, Wen Pan, Fucheng Guo, Jinyu Fu, Zhongyin Liu, Jin Xiao, Shengkui Xu

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引用次数: 0

Abstract

Importance: The Japanese encephalitis virus (JEV) is a massive threat to the pig-raising industry and attenuated-live vaccines are used widely to prevent JEV infections. Primary hamster kidney cells and Vero cells are mainly used to propagate JEV-attenuated vaccines.

Objective: This study aimed to construct porcine mesangial cells (PMCs) for JEV cultivation and study its characteristics.

Methods: An immunofluorescence assay was used to analyze the homogeneity of PMCs. Karyotypic analysis and flow cytometry were used to detect the cell chromosome numbers and cell cycle, respectively. TCID₅₀ was used to measure the viral titers.

Results: The prepared PMCs were uniform in shape and without variation in chromosome numbers. The PMCs exhibit strong division and proliferation ability, and the JEV could replicate efficiently, having a similar cytopathic effect to that in Vero cells. The viral titer results showed that the JEV grows faster in PMCs than in Vero cells.

Conclusions and relevance: This study generated an immortalized PMC cell line suitable for cultivating the JEV, which has significance for producing pig JEV-attenuated vaccines.

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重要性：日本脑炎病毒（JEV）是养猪业的一大威胁，减毒活疫苗被广泛用于预防 JEV 感染。原代仓鼠肾细胞和 Vero 细胞主要用于繁殖 JEV 减毒疫苗：本研究旨在构建用于培养 JEV 的猪肾间质细胞（PMCs）并研究其特性：方法：采用免疫荧光法分析 PMCs 的均一性。核型分析和流式细胞术分别用于检测细胞染色体数目和细胞周期。TCID₅₀用于测量病毒滴度：结果：制备的 PMC 细胞形状均匀，染色体数目无差异。结果：制备的 PMCs 形状均匀，染色体数目无差异，具有很强的分裂和增殖能力，JEV 可有效复制，其细胞病理效应与 Vero 细胞相似。病毒滴度结果显示，JEV在PMCs中的生长速度快于Vero细胞：本研究产生了一种适合培养 JEV 的永生化 PMC 细胞系，对生产猪 JEV 减毒疫苗具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Science 农林科学-兽医学

CiteScore

3.10

自引率

5.60%

发文量

审稿时长

1.3 months

期刊介绍： The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.