Mapping and characterization of a novel powdery mildew resistance locus (PM2) in Cannabis sativa L.

IF 4.1 2区生物学 Q1 PLANT SCIENCES

Frontiers in Plant Science Pub Date : 2025-03-13 eCollection Date: 2025-01-01 DOI:10.3389/fpls.2025.1543229

Soren Seifi, Keegan M Leckie, Ingrid Giles, Taylor O'Brien, John O MacKenzie, Marco Todesco, Loren H Rieseberg, Gregory J Baute, Jose M Celedon

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引用次数: 0

Abstract

Introduction: Breeding genetic resistance to economically important crop diseases is the most sustainable strategy for disease management and enhancing agricultural and horticultural productivity, particularly where the application of synthetic pesticides is prohibited. Powdery mildew disease, caused by the biotrophic fungal pathogen Golovinomyces ambrosiae, is one of the most prevalent threats to the cannabis and hemp industry worldwide.

Methods: In this study, we used bulked-segregant analysis combined with high-throughput RNA sequencing (BSRSeq) to identify and map a novel single dominant resistance (R) locus (designated PM2), that strongly suppresses powdery mildew infection and sporulation in Cannabis sativa.

Results and discussion: BSA mapped PM2 to chromosome 9. Histochemical analysis revealed that PM2-induced resistance is mediated by a highly localized hypersensitive response mainly in the epidermal cells of the host. Importantly, genetic markers capable of tracking PM2 resistance in breeding populations were developed using associated SNPs identified in this study. The ability to track PM2 will allow for successful introgression of PM resistance into elite cannabis cultivars and help move towards a more sustainable cannabis industry.

查看原文本刊更多论文

大麻抗白粉病新位点（PM2）的定位与鉴定。

导言：培育对重要经济作物病害的遗传抗性是病害管理和提高农业及园艺生产力的最可持续战略，尤其是在禁止使用合成杀虫剂的地方。由生物营养真菌病原体 Golovinomyces ambrosiae 引起的白粉病是全球大麻和大麻产业面临的最普遍威胁之一：在这项研究中，我们利用大量分离分析结合高通量 RNA 测序（BSRSeq），鉴定并绘制了一个新的单显性抗性（R）基因座（命名为 PM2），该基因座能强烈抑制大麻白粉病的感染和孢子的产生：BSA 将 PM2 映射到第 9 号染色体上。组织化学分析表明，PM2 诱导的抗性主要是由宿主表皮细胞的高度局部超敏反应介导的。重要的是，利用本研究中发现的相关 SNP，开发出了能够在育种群体中追踪 PM2 抗性的遗传标记。跟踪 PM2 的能力将有助于成功地将 PM 抗性引入精英大麻栽培品种，并帮助大麻产业实现更可持续的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Plant Science PLANT SCIENCES-

CiteScore

7.30

自引率

14.30%

发文量

4844

审稿时长

14 weeks

期刊介绍： In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches. Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.