Haoxin He, Ke Yi, Lei Yang, Yongfeng Jing, Lifu Kang, Zhihao Gao, Dong Xiang, Ge Tan, Yunsheng Wang, Qian Liu, Lin Xie, Shiya Jiang, Tianbo Liu, Wu Chen
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引用次数: 0
Abstract
Introduction: Bacterial wilt (BW) caused by Ralstonia pseudosolanacearum is a devastating soil-borne disease. Bacteriophages are important biocontrol resources that rapidly and specifically lyse host bacteria, showing good application potential in agricultural production.
Methods: This study isolated nine phages (YL1-YL9) and, using host range and pot experiments, identified two broader host range phages (YL1 and YL4) and two higher control efficacy phages (YL2 and YL3), which were combined to obtain five cocktails (BPC-1-BPC-5).
Results: Pot experiments showed that BPC-1 (YL3 and YL4) had the highest control efficacy (99.25%). Biological characterization revealed that these four phages had substantial thermal stability and pH tolerance. Whole genome sequencing and analysis showed that YL1, YL2, YL3, and YL4 belonged to the genus Gervaisevirus. AlphaFold 3 predictions of tail fiber protein II structures showed that YL1 differed significantly from the other phages. Amino acid sequence alignment revealed that the ORF66 (YL1) "tip domain" of contained a higher proportion of aromatic and positively charged amino acids. However, the surface of the ORF69 (YL4) "tip domain" exhibited more positively charged residues than ORF66 (YL2) and ORF70 (YL3). These characteristics are hypothesized to confer a broader host range to YL1 and YL4.
Discussion: This study demonstrates that phages assembling a broad host range and high control efficacy have better biocontrol potential, providing high-quality resources for the biological control of BW.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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