Comprehensive analysis and validation of autophagy-related gene in rheumatoid arthritis.

IF 4.6 2区生物学 Q2 CELL BIOLOGY

Frontiers in Cell and Developmental Biology Pub Date : 2025-03-20 eCollection Date: 2025-01-01 DOI:10.3389/fcell.2025.1563911

Runrun Zhang, Wenhan Huang, Ting Zhao, Jintao Fang, Cen Chang, Dongyi He, Xinchang Wang

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引用次数: 0

Abstract

Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease in which autophagy is pivotal in its pathogenesis. This study aims to identify autophagy-related genes associated with RA and investigate their functional roles.

Methods: We performed mRNA sequencing to identify differentially expressed genes (DEGs) between RA and osteoarthritis (OA) and intersected these with autophagy-related genes to obtain autophagy-related DEGs (ARDEGs) in RA. Bioinformatics and machine learning approaches were used to identify key biomarkers. Functional experiments, including real-time cellular analysis (RTCA), scratch healing, and flow cytometry, were conducted to examine the effects of gene silencing on the proliferation and migration of MH7A cells.

Results: A total of 37 ARDEGs were identified in RA. Through bioinformatics analysis, interferon regulatory factor 4 (IRF4) emerged as a key hub gene, with its high expression confirmed in RA synovial tissues and RA FLS cells. IRF4 knockdown inhibited the proliferation and migration and promoted the death of MH7A cells.

Conclusion: IRF4 is an autophagy-related diagnostic biomarker for RA. Targeting IRF4 could serve as a potential diagnostic and therapeutic strategy for RA, although further clinical studies are required to validate its effectiveness.

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类风湿关节炎自噬相关基因的综合分析与验证。

背景：类风湿性关节炎（RA）是一种慢性自身免疫性疾病，自噬在其发病机制中起关键作用。本研究旨在鉴定与类风湿性关节炎相关的自噬相关基因并探讨其功能作用。方法：我们进行mRNA测序，鉴定RA和骨关节炎（OA）之间的差异表达基因（DEGs），并将其与自噬相关基因交叉，获得RA中自噬相关DEGs （ARDEGs）。使用生物信息学和机器学习方法来识别关键的生物标志物。通过实时细胞分析（RTCA）、划痕愈合和流式细胞术等功能实验，研究基因沉默对MH7A细胞增殖和迁移的影响。结果：在RA中共鉴定出37个ardeg。通过生物信息学分析，干扰素调节因子4 （IRF4）作为关键枢纽基因，在RA滑膜组织和RA FLS细胞中高表达。IRF4敲低抑制MH7A细胞的增殖和迁移，促进其死亡。结论：IRF4是RA自噬相关的诊断生物标志物。靶向IRF4可以作为RA的潜在诊断和治疗策略，尽管需要进一步的临床研究来验证其有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Cell and Developmental Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

9.70

自引率

3.60%

发文量

2531

审稿时长

12 weeks

期刊介绍： Frontiers in Cell and Developmental Biology is a broad-scope, interdisciplinary open-access journal, focusing on the fundamental processes of life, led by Prof Amanda Fisher and supported by a geographically diverse, high-quality editorial board. The journal welcomes submissions on a wide spectrum of cell and developmental biology, covering intracellular and extracellular dynamics, with sections focusing on signaling, adhesion, migration, cell death and survival and membrane trafficking. Additionally, the journal offers sections dedicated to the cutting edge of fundamental and translational research in molecular medicine and stem cell biology. With a collaborative, rigorous and transparent peer-review, the journal produces the highest scientific quality in both fundamental and applied research, and advanced article level metrics measure the real-time impact and influence of each publication.