Transboundary intercellular communication mechanisms in the treatment of polyvinyl alcohol (PVA) wastewater by Geotrichum candidume enhanced activated sludge

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-04-05 DOI:10.1016/j.watres.2025.123529

Rui Zhang, Sixin Zhang, Chunrui Li, Bin Cui, Dandan Zhou

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Abstract

Polyvinyl alcohol (PVA) wastewater is characterized by low biochemistry and poor biodegradability. Transboundary intercellular communication between fungi and bacteria modulates microbial metabolism activity. This mechanism facilitates the biological treatment of PVA wastewater potentially. In this study, a Geotrichum candidume enhanced activated sludge system was developed to treat PVA wastewater. The transboundary intercellular communication mechanism among fungi and bacteria was elucidated, and the promotion of PVA removal was studied. Results showed that G. geotrichum sensed the N-decanoyl-l-homoserine lactone (C10-HSL) secreted by Acidovorax, leading to the upregulation of functional genes (HAO, LACC1, FAHD) for PVA degradation. Dehydrogenase (DHA) and laccase activities were increased by 15.2 %-38.6 % and 77.1 %-114.5 % after C10-HSL addition. Meanwhile, C10-HSL enhanced metabolic processes such as tricarboxylic acid cycle (TCA cycle), and oxidative phosphorylation in G. geotrichum to accelerate PVA degradation. As a result, G. geotrichum biodegraded PVA into aldehydes and ketones, which were further mineralized by bacteria such as Dechloromonas. For the co-culture system, the synergistic interaction between G. geotrichum and functional bacteria significantly enhanced PVA degradation via the intercellular communication process. The PVA and chemical oxygen demand (COD) removal increased by 31.7 % and 28.3 %, respectively, compared to the activated sludge system. This study offers a novel theoretical foundation and approach for the biological treatment of PVA wastewater through fungal-bacterial transboundary intercellular communication.

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.