An organic small molecule fluorescent probe for nondestructive detection of Zn2+ in plants

IF 1.5 4区化学 Q3 CHEMISTRY, ORGANIC

Tetrahedron Letters Pub Date : 2025-03-21 DOI:10.1016/j.tetlet.2025.155553

Junyi Zheng , Siyi Li , Shunpeng Bai , Xiao Liu , Ruitao Sun , Shengzhen Xu , Wei Qu

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Abstract

In this study, a novel two-photon fluorescent probe, ZT1, leveraging the photoinduced electron transfer (PET) effect was designed and synthesized. The probe employs 4-trifluoromethyl coumarin as the fluorophore and 2,2′-dipicolylamine (DPA) as the Zn²⁺ recognition group, enabling non-destructive detection of Zn²⁺ in Arabidopsis thaliana. ZT1 demonstrated exceptional selectivity for Zn²⁺ in both in vitro and in vivo assays, maintained stability across a pH range of 6–8, featured a detection limit as low as 13.96 nM, and exhibited a large Stokes shift (177 nm). The probe facilitated high-resolution visualization of Zn²⁺ in the root tips and leaf tissues of Arabidopsis thaliana and demonstrated remarkable reversibility in cyclic experiments. This work establishes a robust toolkit that overcomes longstanding limitations in plant metal ion imaging, offering mechanistic insights into zinc-regulated physiological processes.

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一种无损检测植物中Zn2+的有机小分子荧光探针

本研究设计并合成了一种利用光诱导电子转移效应（PET）的新型双光子荧光探针 ZT1。该探针采用 4-三氟甲基香豆素作为荧光团，2,2′-二羟基胺（DPA）作为 Zn2+ 识别基团，实现了拟南芥中 Zn2+ 的无损检测。ZT1 在体外和体内检测中都表现出对 Zn2+ 的卓越选择性，在 pH 值为 6-8 的范围内保持稳定，检测限低至 13.96 nM，并表现出较大的斯托克斯偏移（177 nm）。该探针有助于拟南芥根尖和叶片组织中 Zn2+ 的高分辨率可视化，并在循环实验中表现出显著的可逆性。这项工作建立了一个强大的工具包，克服了植物金属离子成像中长期存在的局限性，为锌调控的生理过程提供了机理上的见解。

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来源期刊

Tetrahedron Letters 化学-有机化学

CiteScore

3.50

自引率

5.60%

发文量

521

审稿时长

28 days

期刊介绍： Tetrahedron Letters provides maximum dissemination of outstanding developments in organic chemistry. The journal is published weekly and covers developments in techniques, structures, methods and conclusions in experimental and theoretical organic chemistry. Rapid publication of timely and significant research results enables researchers from all over the world to transmit quickly their new contributions to large, international audiences.