Residue-specific protein-glycan conjugation strategies for the development of pharmaceutically promising glycoconjugate vaccines: A recent update

Abstract

Covalent coupling between a carbohydrate antigen and a protein carrier leads to the formation of pharmaceutically promising glycoconjugate vaccines. Most licensed glycoconjugate vaccines are acquired by random bioconjugation of native or sized glycans with the surface-exposed amino acid residues of proteins, such as lysine, cysteine, aspartic acid, glutamic amino acid, etc. In the last two decades, considerable momentum has been gained in the glycoconjugate vaccine development by discovering several residue-specific bioconjugation strategies. As a result, glycoconjugate chemistry reaches the verge of discovering well-defined and “real” homogeneous vaccines, which may be more potent to generate antimicrobial resistance against “bad-bugs”. Through this literature survey, we intend to highlight the state of the art of residue-specific bioconjugation of proteins with glycans to obtain glycoconjugate vaccines. The review will also identify a potential roadmap to address the gap and the prospects in the medicinal domain.