{"title":"Localization of the active principles of the male fern (Dryopteris filixmas (L.) Schott.) by fluorescence microscopy","authors":"G.G. Franchi, S. Ferri","doi":"10.1016/S0031-6989(88)80858-0","DOIUrl":null,"url":null,"abstract":"<div><p>The active principles of the male fern, <em>Dryopteris filix-mas</em> (L.) Schott., consisting of a mixture of phloroglucinol derivatives called “crude filicin”, are localized by means of fluorescence microscopy under U.V. 365 nm and monochromatic blue 435-490 nm lights.</p><p>Crude filicin is autofluorescent, and therefore may be easily localized in the internal glandular hairs of the intercellular spaces of rhizome and leaf bases parenchyma, without pre-treatment of sections.</p><p>Localization is confirmed both morphologically and chemically, and is possible on a fresh material, as well as on a desiccated one.</p><p>The test allows also a complete anatomical examination of sections, and may be useful, for its easiness and quickness, in further taxonomic or pharmacognostic studies.</p></div>","PeriodicalId":19810,"journal":{"name":"Pharmacological research communications","volume":"20 ","pages":"Pages 135-138"},"PeriodicalIF":0.0000,"publicationDate":"1988-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0031-6989(88)80858-0","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological research communications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0031698988808580","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
The active principles of the male fern, Dryopteris filix-mas (L.) Schott., consisting of a mixture of phloroglucinol derivatives called “crude filicin”, are localized by means of fluorescence microscopy under U.V. 365 nm and monochromatic blue 435-490 nm lights.
Crude filicin is autofluorescent, and therefore may be easily localized in the internal glandular hairs of the intercellular spaces of rhizome and leaf bases parenchyma, without pre-treatment of sections.
Localization is confirmed both morphologically and chemically, and is possible on a fresh material, as well as on a desiccated one.
The test allows also a complete anatomical examination of sections, and may be useful, for its easiness and quickness, in further taxonomic or pharmacognostic studies.
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