Miao Yu, Siqin Wang, Lingdie Kong, Mengsha Huang, Jin Zhang, Jing Li, Ruohan Wang
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引用次数: 0
Abstract
Floral thermogenesis is an ancient feature that facilitates mutualism between flowers and pollinators. Yet localization of specific thermogenic tissues within floral organs has received little attention. Here, we integrated infrared (IR) thermal imaging and micro X-ray fluorescence (μ-XRF) to localize the thermogenic tissues in the lotus (Nelumbo nucifera Gaertn.) receptacle. IR imaging preliminarily identified the primary thermogenic tissues of the receptacle as the carpels and epidermis. The calcium distribution visualized by μ-XRF complemented the results of IR imaging, indicating that the thermogenic tissues include the epidermis and the upper parts of the carpels. This ensures that heat reaches the chamber formed by the petals and receptacle over the shortest distance, thereby minimizing heat loss. Additionally, we observed a higher rate of Ca2+ transport from the apoplast to the cytosol and upregulation of genes associated with mitochondrial calcium uniporters (MCU) at the thermogenesis initiation stage as compared to the pre-thermogenic stage. Increasing the cytosolic Ca2+ (cCa2+) concentration reversed the inhibition of alternative respiratory pathways, further illustrating the close relationship between Ca2+ concentration and thermogenesis. Our research not only presents a precise method for identifying thermogenic tissues in plants but also demonstrates the evolutionary efforts of lotus to maximize energy utilization efficiency.
期刊介绍:
Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research.
As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.