Multiscale Imaging Locates Thermogenic Tissues and Reveals the Role of Ca2+ in Floral Thermogenesis

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Miao Yu, Siqin Wang, Lingdie Kong, Mengsha Huang, Jin Zhang, Jing Li, Ruohan Wang
{"title":"Multiscale Imaging Locates Thermogenic Tissues and Reveals the Role of Ca2+ in Floral Thermogenesis","authors":"Miao Yu, Siqin Wang, Lingdie Kong, Mengsha Huang, Jin Zhang, Jing Li, Ruohan Wang","doi":"10.1093/plphys/kiaf131","DOIUrl":null,"url":null,"abstract":"Floral thermogenesis is an ancient feature that facilitates mutualism between flowers and pollinators. Yet localization of specific thermogenic tissues within floral organs has received little attention. Here, we integrated infrared (IR) thermal imaging and micro X-ray fluorescence (μ-XRF) to localize the thermogenic tissues in the lotus (Nelumbo nucifera Gaertn.) receptacle. IR imaging preliminarily identified the primary thermogenic tissues of the receptacle as the carpels and epidermis. The calcium distribution visualized by μ-XRF complemented the results of IR imaging, indicating that the thermogenic tissues include the epidermis and the upper parts of the carpels. This ensures that heat reaches the chamber formed by the petals and receptacle over the shortest distance, thereby minimizing heat loss. Additionally, we observed a higher rate of Ca2+ transport from the apoplast to the cytosol and upregulation of genes associated with mitochondrial calcium uniporters (MCU) at the thermogenesis initiation stage as compared to the pre-thermogenic stage. Increasing the cytosolic Ca2+ (cCa2+) concentration reversed the inhibition of alternative respiratory pathways, further illustrating the close relationship between Ca2+ concentration and thermogenesis. Our research not only presents a precise method for identifying thermogenic tissues in plants but also demonstrates the evolutionary efforts of lotus to maximize energy utilization efficiency.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"16 1","pages":""},"PeriodicalIF":6.5000,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/plphys/kiaf131","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Floral thermogenesis is an ancient feature that facilitates mutualism between flowers and pollinators. Yet localization of specific thermogenic tissues within floral organs has received little attention. Here, we integrated infrared (IR) thermal imaging and micro X-ray fluorescence (μ-XRF) to localize the thermogenic tissues in the lotus (Nelumbo nucifera Gaertn.) receptacle. IR imaging preliminarily identified the primary thermogenic tissues of the receptacle as the carpels and epidermis. The calcium distribution visualized by μ-XRF complemented the results of IR imaging, indicating that the thermogenic tissues include the epidermis and the upper parts of the carpels. This ensures that heat reaches the chamber formed by the petals and receptacle over the shortest distance, thereby minimizing heat loss. Additionally, we observed a higher rate of Ca2+ transport from the apoplast to the cytosol and upregulation of genes associated with mitochondrial calcium uniporters (MCU) at the thermogenesis initiation stage as compared to the pre-thermogenic stage. Increasing the cytosolic Ca2+ (cCa2+) concentration reversed the inhibition of alternative respiratory pathways, further illustrating the close relationship between Ca2+ concentration and thermogenesis. Our research not only presents a precise method for identifying thermogenic tissues in plants but also demonstrates the evolutionary efforts of lotus to maximize energy utilization efficiency.
求助全文
约1分钟内获得全文 求助全文
来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信