{"title":"Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system.","authors":"Jinzhi Li, He Zhang, Jianming Gao","doi":"10.1016/j.xpro.2025.103707","DOIUrl":null,"url":null,"abstract":"<p><p>A non-invasive system can measure nutrient uptake and photosynthate partitioning simultaneously while allowing organisms to metabolize without interference in the process of measurement. Here, we present a protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system. We describe steps for preparing plant material and material for the non-invasive system. We then detail procedures for setting up and applying the non-invasive measurement system. For complete details on the use and execution of this protocol, please refer to Li et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"6 2","pages":"103707"},"PeriodicalIF":1.3000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"STAR Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xpro.2025.103707","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
A non-invasive system can measure nutrient uptake and photosynthate partitioning simultaneously while allowing organisms to metabolize without interference in the process of measurement. Here, we present a protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system. We describe steps for preparing plant material and material for the non-invasive system. We then detail procedures for setting up and applying the non-invasive measurement system. For complete details on the use and execution of this protocol, please refer to Li et al.1.