{"title":"Identification and characterization of a novel reovirus strain isolated fromgrass carp (Ctenopharyngodon idella).","authors":"Weiguang Kong, Guangyi Ding, Qiushi Zhang, Xinjie Yuan, Yuchao Zhu, Liyuan Ma, Chang Cai, Yong Shi, Qianqian Zhang, Zhen Xu","doi":"10.1186/s12985-025-02716-8","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Grass carp (Ctenopharyngodon idella) hemorrhagic disease (GCHD) is a devastating disease that leads to substantial economic losses in the freshwater aquaculture industry.</p><p><strong>Results: </strong>In this study, we investigated an outbreak of GCHD in large-scale grass carp and identified GCRV-II infection. Notably, hematoxylin and eosin (H&E) staining showed severe histopathological changes in the spleen, head kidney, gill, and gut. Furthermore, we sequenced the entire genome of the viral isolate, and multiple sequence alignment and phylogenetic tree analysis indicated that it represents a novel strain of GCRV-II, provisionally named GCRV-YX246. Finally, artificial infection experiments confirmed the strong virulence, high mortality, and severe pathological damage caused by GCRV-YX246, as demonstrated through artificial infection.</p><p><strong>Conclusions: </strong>A novel reovirus from large-scale grass carp cultured in China was identified. The discovery of this novel GCRV-II strain enhances our understanding of GCRV-II biology and provides valuable insights for developing more effective prevention strategies for GCHD.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"92"},"PeriodicalIF":4.0000,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12985-025-02716-8","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Grass carp (Ctenopharyngodon idella) hemorrhagic disease (GCHD) is a devastating disease that leads to substantial economic losses in the freshwater aquaculture industry.
Results: In this study, we investigated an outbreak of GCHD in large-scale grass carp and identified GCRV-II infection. Notably, hematoxylin and eosin (H&E) staining showed severe histopathological changes in the spleen, head kidney, gill, and gut. Furthermore, we sequenced the entire genome of the viral isolate, and multiple sequence alignment and phylogenetic tree analysis indicated that it represents a novel strain of GCRV-II, provisionally named GCRV-YX246. Finally, artificial infection experiments confirmed the strong virulence, high mortality, and severe pathological damage caused by GCRV-YX246, as demonstrated through artificial infection.
Conclusions: A novel reovirus from large-scale grass carp cultured in China was identified. The discovery of this novel GCRV-II strain enhances our understanding of GCRV-II biology and provides valuable insights for developing more effective prevention strategies for GCHD.
期刊介绍：
Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies.
The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.
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