{"title":"<i>Calendula officinalis</i> extract enhances wound healing by promoting fibroblast activity and reducing inflammation in mice.","authors":"Yalcin Alper Ozturan, Ibrahim Akin","doi":"10.1080/15569527.2025.2485129","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Despite evidence supporting the therapeutic potential of <i>Calendula officinalis</i>, well-designed and controlled studies are still needed to confirm its beneficial effects on various health conditions, including skin care. This study therefore evaluates the effectiveness of topically administered 5% aqueous <i>Calendula officinalis</i> extract on healing full-thickness skin wounds in male BALB/c mice.</p><p><strong>Methods: </strong>Seventy-two mice were divided into three groups: CAL (treated with calendula extract), PSS (treated with physiological saline), and NC (negative control/no treatment). Wound healing was assessed over 14 days using planimetric measurements, counting fibroblasts and macrophages, biochemical analyses of growth factors, inflammation markers, hydroxyproline levels, and genomic analyses.</p><p><strong>Results: </strong>The data obtained show that the application of CAL extract significantly reduces wound areas by day 7 compared to the NC and PSS groups. CAL extract also leads to an increase in fibroblasts, fibroblast growth factor, and hydroxyproline levels, while it reduces macrophages and inflammatory biomarkers levels in the healing wound. Genomic analyses indicate that topical application of CAL extract significantly reduces the expression of inflammatory biomarkers, including matrix metalloproteinases 2 and 9.</p><p><strong>Conclusions: </strong>These findings show that 5% aqueous CAL extract enhances wound healing promising new insights for the effective topical treatment of skin wounds.</p>","PeriodicalId":11023,"journal":{"name":"Cutaneous and Ocular Toxicology","volume":" ","pages":"1-11"},"PeriodicalIF":1.6000,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cutaneous and Ocular Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/15569527.2025.2485129","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: Despite evidence supporting the therapeutic potential of Calendula officinalis, well-designed and controlled studies are still needed to confirm its beneficial effects on various health conditions, including skin care. This study therefore evaluates the effectiveness of topically administered 5% aqueous Calendula officinalis extract on healing full-thickness skin wounds in male BALB/c mice.
Methods: Seventy-two mice were divided into three groups: CAL (treated with calendula extract), PSS (treated with physiological saline), and NC (negative control/no treatment). Wound healing was assessed over 14 days using planimetric measurements, counting fibroblasts and macrophages, biochemical analyses of growth factors, inflammation markers, hydroxyproline levels, and genomic analyses.
Results: The data obtained show that the application of CAL extract significantly reduces wound areas by day 7 compared to the NC and PSS groups. CAL extract also leads to an increase in fibroblasts, fibroblast growth factor, and hydroxyproline levels, while it reduces macrophages and inflammatory biomarkers levels in the healing wound. Genomic analyses indicate that topical application of CAL extract significantly reduces the expression of inflammatory biomarkers, including matrix metalloproteinases 2 and 9.
Conclusions: These findings show that 5% aqueous CAL extract enhances wound healing promising new insights for the effective topical treatment of skin wounds.
期刊介绍:
Cutaneous and Ocular Toxicology is an international, peer-reviewed journal that covers all types of harm to cutaneous and ocular systems. Areas of particular interest include pharmaceutical and medical products; consumer, personal care, and household products; and issues in environmental and occupational exposures.
In addition to original research papers, reviews and short communications are invited, as well as concise, relevant, and critical reviews of topics of contemporary significance.