Formulation optimization and characterization of Central composite design optimized LAP-loaded calcium pectinate nanoparticles: anticancer activity against MCF-7 cells.

Abstract

Objective: The study aimed to formulate and optimize Lapatinib-loaded calcium pectinate nanoparticles (LAP-PEC-NPs) using central composite design (CCD), evaluate their physicochemical properties, and compare their anticancer efficacy with raw LAP on MCF7 breast cancer cell lines.

Significance of review: The study is significant as it successfully developed LAP-PEC-NPs through systematic optimization. These nanoparticles exhibited favorable physicochemical properties, high drug entrapment, and sustained release. The effective inhibition of MCF7 breast cancer cell growth by LAP-PEC-NPs underscores their potential as a promising cancer treatment strategy, enhancing LAP's therapeutic efficacy and bioavailability.

Key findings: LAP-PEC-NPs were successfully developed using an ionic gelation process. The optimization resulted in an ideal formulation with a polydispersity index (PDI) of 0.289, a droplet size of 93.65 nm, and a zeta potential of -17.32 mV. LAP's amorphous nature within the nanoparticles' porous matrix was confirmed through characterization techniques. Dissolution studies showed sustained drug release, with LAP-PEC-NPs releasing approximately 75% of LAP over 72 h, significantly higher than raw LAP. Evaluation of MCF7 breast cancer cell lines revealed that LAP-PEC-NPs effectively inhibited cell growth.

Conclusions: The study successfully developed and optimized LAP-PEC-NPs, yielding nanoparticles with desirable characteristics. The sustained drug release kinetics and promising anticancer efficacy of LAP-PEC-NPs suggest their potential as a therapeutic strategy for breast cancer treatment. These findings pave the way for further preclinical and clinical studies to validate the efficacy and safety of LAP-PEC-NPs for clinical translation.