Tartrate-Resistant Acid Phosphatase 5 (TRAP5) Promotes Eosinophil Migration During Allergic Asthma.

IF 5.9 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Médea Padra, Jesper Bergwik, Anna Adler, Genevieve Marcoux, Ravi K V Bhongir, Praveen Papareddy, Arne Egesten
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引用次数: 0

Abstract

Allergic asthma is characterized by type 2 inflammation and eosinophilia. Tartrate-resistant acid phosphatase 5 (TRAP5/ACP5) is a metallophosphatase expressed by alveolar macrophages that dephosphorylates osteopontin (OPN), a phosphoglycoprotein with increased expression in asthma. To investigate the role of TRAP5 during asthma, we used a murine model of ovalbumin (OVA) induced allergic airway inflammation as well as IL-33 induced airway inflammation including Trap5-/- and wild-type (WT) mice. Histological analyses of murine lung revealed that OVA-induced inflammation induced the formation of inflammatory lesions and increased mucus production in both WT and Trap5-/- mice. However, lower cytokine levels (including IL-5 and IL-13) were detected by multiplex immunoassay in Trap5-/- mice after OVA-induced inflammation. Furthermore, qPCR analysis detected different gene expression profile of Trap5-/-/OVA mice, including upregulation of Il-17a and downregulation of Il-33. Lower eosinophil numbers were measured in bronchoalveolar lavage fluid of Trap5-/-/OVA mice using flow cytometry analysis, whereas immunofluorescence staining revealed high eosinophil number in lung tissue of both groups with OVA challenge. In the IL-33 model of type 2 inflammation, both WT and Trap5-/- mice showed similar inflammatory responses with regard to cytokine levels and cell recruitment patterns. In vitro, eosinophil chemotaxis was facilitated by non-phosphorylated but not phosphorylated OPN, an effect inhibited by an α4β1 integrin inhibitor. The results suggest that TRAP5 is important in the recruitment of immune cells, including eosinophils, as well as in shaping the profile and amplification of the inflammatory response during allergic airway inflammation. Thus, TRAP5 may serve as a therapeutic target in allergic asthma. This article is open access and distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).

抗酒石酸酸性磷酸酶5 (TRAP5)在过敏性哮喘中促进嗜酸性粒细胞迁移。
过敏性哮喘的特点是2型炎症和嗜酸性粒细胞增多。抗酒石酸酸性磷酸酶5 (TRAP5/ACP5)是一种由肺泡巨噬细胞表达的金属磷酸酶,可使骨桥蛋白(OPN)去磷酸化,骨桥蛋白是一种在哮喘中表达增加的磷酸糖蛋白。为了研究TRAP5在哮喘中的作用,我们使用了卵清蛋白(OVA)诱导的过敏性气道炎症和IL-33诱导的气道炎症小鼠模型,包括TRAP5 -/-和野生型(WT)小鼠。小鼠肺组织分析显示,ova诱导的炎症在WT和Trap5-/-小鼠中均诱导炎性病变的形成和粘液产生的增加。然而,在ova诱导炎症后的Trap5-/-小鼠中,多重免疫分析法检测到较低的细胞因子水平(包括IL-5和IL-13)。此外,qPCR检测到Trap5-/-/OVA小鼠的不同基因表达谱,包括Il-17a上调和Il-33下调。流式细胞术检测Trap5-/-/OVA小鼠支气管肺泡灌洗液中嗜酸性粒细胞数量较低,而免疫荧光染色显示两组OVA小鼠肺组织中嗜酸性粒细胞数量均较高。在2型炎症的IL-33模型中,WT和Trap5-/-小鼠在细胞因子水平和细胞募集模式方面表现出相似的炎症反应。在体外,非磷酸化而非磷酸化的OPN促进了嗜酸性粒细胞趋化,α4β1整合素抑制剂抑制了这一作用。结果表明,TRAP5在免疫细胞(包括嗜酸性粒细胞)的募集以及过敏性气道炎症期间炎症反应的形成和放大中发挥重要作用。因此,TRAP5可能作为过敏性哮喘的治疗靶点。本文在知识共享署名4.0国际许可协议(https://creativecommons.org/licenses/by/4.0/)的条款下开放获取和分发。
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来源期刊
CiteScore
11.20
自引率
3.10%
发文量
370
审稿时长
3-8 weeks
期刊介绍: The American Journal of Respiratory Cell and Molecular Biology publishes papers that report significant and original observations in the area of pulmonary biology. The focus of the Journal includes, but is not limited to, cellular, biochemical, molecular, developmental, genetic, and immunologic studies of lung cells and molecules.
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