The Impact of Channel Geometry and Flow Regime on Endothelial Orientation and Morphology in Vessel-on-Chip

IF 6.4 3区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Mohammad Jouybar, Sophie van der Kallen, Sheen Sahebali, Carlijn Bouten, Jaap M.J. den Toonder
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Abstract

This study investigates the impact of channel geometry and applied flow on the orientation and morphology of endothelial cells (ECs) within vessel-on-chip (VoC) models. Traditional organ-on-chip models often utilize rectangular cross-section channels, resulting in flat walls, sharp corners, and non-uniform wall shear stress profiles, which do not accurately mimic physiological conditions. Tubular channels with circular cross-sections provide a more in vivo-like geometry and result in a physiological uniform wall shear stress. Here, tubular channels fabricated using sugar 3D-printing are compared with rectangular channels made via stereolithography 3Dprinting. The results show that ECs from both blood and lymph vessels exhibit more uniform coverage and circumferential alignment in tubular channels than in rectangular channels. Unidirectional or bidirectional flow conditions align ECs parallel to the flow, overruling the circumferential alignment induced by curvature in tubular channels. Pulsatile flow enhances circumferential orientation in tubular channels, while alignment along the flow is maintained in rectangular channels. Additionally, EC orientation induced by flow impacts monocyte rolling velocities, crucial for understanding immune cell motility. This study underlines the importance of the combined effect of channel geometry and flow conditions in VoC models, and advocates for the continued development of advanced organ-on-chip systems that better replicate human physiology.

Abstract Image

血管芯片中通道几何形状和流动状态对内皮取向和形态的影响
本研究探讨了通道几何形状和应用流量对血管芯片(VoC)模型中内皮细胞(ECs)的取向和形态的影响。传统的器官芯片模型通常使用矩形的横截面通道,导致壁平,角尖,壁剪应力分布不均匀,不能准确模拟生理条件。具有圆形截面的管状通道提供了更类似于体内的几何形状,并导致生理均匀的壁剪切应力。在这里,用糖3d打印制造的管状通道与用立体光刻3d打印制造的矩形通道进行了比较。结果表明,与矩形通道相比,来自血管和淋巴管的内皮细胞在管状通道中表现出更均匀的覆盖和周向排列。单向或双向流动条件使ec平行于流动,而不是管状通道中曲率引起的周向排列。在管状通道中,脉动流增强了环向定向,而在矩形通道中则保持了沿流动方向的定向。此外,流动诱导的EC取向影响单核细胞滚动速度,这对于理解免疫细胞运动至关重要。这项研究强调了通道几何形状和流动条件在VoC模型中的综合影响的重要性,并倡导继续发展先进的器官芯片系统,以更好地复制人体生理学。
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来源期刊
Advanced Materials Technologies
Advanced Materials Technologies Materials Science-General Materials Science
CiteScore
10.20
自引率
4.40%
发文量
566
期刊介绍: Advanced Materials Technologies Advanced Materials Technologies is the new home for all technology-related materials applications research, with particular focus on advanced device design, fabrication and integration, as well as new technologies based on novel materials. It bridges the gap between fundamental laboratory research and industry.
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