Analysis of Volatile Organic Compounds Differences of Black and White Pepper by HS-GC-IMS and HS-SPME-GC-MS

IF 1.8 3区 化学 Q4 BIOCHEMICAL RESEARCH METHODS
Xue Chen, Jiaxin Yin, Yang Zhang, Jiaxuan Chen, Songtao Bie, Xinbo Song, Zheng Li, Liping Kang, Heshui Yu
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引用次数: 0

Abstract

Rationale

As a medicinal and edible herb, Piper nigrum L. is abundant in volatile organic compounds (VOCs), and its essential oil has antibacterial properties. Notably, the aromatic profiles of black pepper (BP) and white pepper (WP) are markedly distinct. Consequently, it is essential to comprehensively characterize the VOCs of BP and WP, and analyze the differences in their VOCs and antibacterial efficacy.

Methods

This study analyzed the VOCs of BP and WP using headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). The fingerprint of HS-GC-IMS was established. Random forest analysis, orthogonal partial least squares discriminant analysis and heatmap were used to analyze differences in BP and WP. Furthermore, the antibacterial efficacy of volatile oils derived from BP and WP was assessed using the antibacterial zone diameter method.

Results

A total of 108 and 123 VOCs were identified by HS-GC-IMS and HS-SPME-GC-MS, respectively. The results of multivariate statistical analysis showed that the VOCs of BP and WP are significantly different. In contrast, WP has a milder smell than BP, while BP has a more pungent odor. Eight differential markers were selected. Both BP and WP had inhibitory effects on Staphylococcus aureus and Candida albicans.

Conclusion

This study helps to decipher the flavor differences between BP and WP, and provides a material basis for their quality control and pharmacodynamics. It is beneficial to enhance its utilization within the domains of nutrition and traditional Chinese medicine.

用HS-GC-IMS和HS-SPME-GC-MS分析黑胡椒和白胡椒挥发性有机物的差异
研究原理胡椒属药用和食用草本植物,含有丰富的挥发性有机化合物(VOCs),其精油具有抗菌作用。值得注意的是,黑胡椒(BP)和白胡椒(WP)的芳香谱明显不同。因此,有必要对BP和WP的VOCs进行综合表征,并分析其VOCs和抗菌效果的差异。方法采用顶空气相色谱-离子迁移谱法(HS-GC-IMS)和顶空固相微萃取-气相色谱-质谱法(HS-SPME-GC-MS)对BP和WP的挥发性有机化合物进行分析。建立了HS-GC-IMS指纹图谱。采用随机森林分析、正交偏最小二乘判别分析和热图分析BP和WP的差异。采用抑菌带直径法对BP和WP提取的挥发油进行抑菌效果评价。结果HS-GC-IMS和HS-SPME-GC-MS分别鉴定出108种和123种挥发性有机化合物。多元统计分析结果显示,BP和WP的VOCs存在显著差异。相比之下,WP的气味比BP温和,而BP的气味更刺鼻。选择8个鉴别标记。BP和WP对金黄色葡萄球菌和白色念珠菌均有抑制作用。结论本研究有助于揭示BP和WP的风味差异,为其质量控制和药效学研究提供物质依据。有利于提高其在营养和中药领域的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
219
审稿时长
2.6 months
期刊介绍: Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.
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