Deep Learning-Powered Colloidal Digital SERS for Precise Monitoring of Cell Culture Media

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-04-03 DOI:10.1021/acs.nanolett.5c01071

Peng Zheng, Lintong Wu, Michael Ka Ho Lee, Andy Nelson, Michael Betenbaugh, Ishan Barman

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引用次数: 0

Abstract

Maintaining consistent quality in biomanufacturing is essential for producing high-quality complex biologics. Yet, current process analytical technologies (PAT) often fall short in achieving rapid and accurate monitoring of small-molecule critical process parameters and critical quality attributes. Surface-enhanced Raman spectroscopy (SERS) holds great promise but faces challenges like intensity fluctuations, compromising reproducibility. Herein, we propose a deep learning-powered colloidal digital SERS platform. This innovation converts SERS spectra into binary “ON/OFF” signals based on defined intensity thresholds, which allows single-molecule event visualization and reduces false positives. Through integration with deep learning, this platform enables detection of a broad range of analytes, unlimited by the lack of characteristic SERS peaks. Furthermore, we demonstrate its accuracy and reproducibility for studying AMBIC 1.1 mammalian cell culture media. These results highlight its rapidity, accuracy, and precision, paving the way for widespread adoption and scale-up as a novel PAT tool in biomanufacturing and diagnostics.

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.