Highly accurate multiplexed nanoplasmonic detection of microRNAs using splinted ligation

IF 13.2 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-04-02 DOI:10.1016/j.cej.2025.162100

Yun Ju Sung, Sihwa Joo, Ui Jin Lee, Ki joong Lee, Jaejong Lee, Na rae Jo, Yong-Beom Shin, Mina Lee

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引用次数: 0

Abstract

MicroRNAs (miRNAs) are short single-stranded RNAs that regulate gene expression at the post-transcriptional level. Abnormal expression of miRNAs is widely observed in cancer; hence, miRNAs have been recognized as important cancer biomarkers. However, the accurate detection of miRNAs is challenging owing to their short length and high sequence homology. In this study, we developed an accurate multiplexed miRNA detection method based on localized surface plasmonic resonance (LSPR) of metallic nanostructures. MiRNAs were labeled with DNA probes by splinted ligation and immobilized on a gold nanodot array. After further intensification via enzyme-assisted precipitation, the shift in LSPR absorption was measured to estimate the concentration of miRNAs. Three miRNAs (miR-34c, miR-99a, and miR-125b) were detected with very high sensitivity (limit of detection: 18–261 zmol). The clear discrimination of single-nucleotide variations and precise estimation of miRNA concentrations demonstrated the high accuracy of this method. The relative abundance of miRNAs in total RNA was also successfully assessed using this method

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使用夹板连接的高精度多路纳米等离子体检测microrna

MicroRNAs （miRNAs）是在转录后水平调控基因表达的短单链rna。miRNAs的异常表达在肿瘤中广泛存在；因此，mirna已被认为是重要的癌症生物标志物。然而，由于mirna长度短，序列同源性高，因此准确检测mirna具有挑战性。在这项研究中，我们开发了一种基于金属纳米结构的局部表面等离子体共振（LSPR）的精确多路miRNA检测方法。用DNA探针通过夹板连接标记mirna，并将其固定在金纳米点阵列上。通过酶辅助沉淀进一步强化后，测量LSPR吸收的变化来估计mirna的浓度。三种mirna （miR-34c， miR-99a和miR-125b）的检测灵敏度非常高（检测限：18-261 zmol）。对单核苷酸变异的清晰区分和对miRNA浓度的精确估计表明该方法具有较高的准确性。使用这种方法也成功地评估了总RNA中mirna的相对丰度

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.