Transcriptome analysis unveils the functional effects of ectomycorrhizal fungal colonization on cadmium tolerance of willow saplings.

IF 4 2区生物学 Q2 MICROBIOLOGY

Frontiers in Microbiology Pub Date : 2025-03-18 eCollection Date: 2025-01-01 DOI:10.3389/fmicb.2025.1570200

Lijiao Wang, Baoshan Yang, Hui Wang, Jiaxing Shi, Jinhao Dong, Xiaoxia Zhao, Guanghua Qin, Xinhua He, Meiyuan Wang

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引用次数: 0

Abstract

Introduction: Ectomycorrhizal fungus (ECMF) could enhance plant tolerance to heavy metal toxicity by altering metal accumulation and protecting plants from oxidative injury. However, the molecular mechanisms underlying ECMF-mediated detoxification of cadmium (Cd) in willow sapling are not well known. This study aimed to unveil the roles of Cenococcum geophilum (CG) and Suillus luteus (SL) in regulating Cd toxicity tolerance in willow (Salix psammophila 'Huangpi1') saplings.

Methods: This study systematically evaluated physiological and biochemical parameters in the leaf and root tissues of 18 willow saplings, while concurrently conducting transcriptomic analysis of the roots under Cd stress. The specific treatments were labeled as follows: NF (no ECMF inoculation and no Cd addition), CG (CG colonization only), SL (SL colonization only), NF+Cd (no ECMF inoculation with 100 μM Cd addition), CG+Cd (CG colonization with 100 μM Cd addition), and SL+Cd (SL colonization with 100 μM Cd addition).

Results: The results showed the growth, photosynthesis, antioxidant system and transcriptome of 2-month-old willow saplings responded differently to ECMFs colonization under Cd stress. S. luteus markedly increased the aerial parts biomass, while C. geophilum significantly enhanced the root property indices of willow saplings under Cd stress. The highest number of differentially expressed genes (DEGs) was observed in the comparison between CG+Cd (CG colonization with 100 μM Cd addition) and NF+Cd (no ECMF inoculation with 100 μM Cd addition). C. geophilum colonization activated plant hormone signal transduction and carbohydrate metabolism pathways, while S. luteus enhanced the synthesis of secondary metabolites.

Discussion: This study provides a molecular perspective on the mechanism of interaction between ECMFs and willow saplings under Cd stress and supports the application of ECMFs for phytoremediation of Cd-contaminated soil.

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来源期刊

Frontiers in Microbiology MICROBIOLOGY-

CiteScore

7.70

自引率

9.60%

发文量

4837

审稿时长

14 weeks

期刊介绍： Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.