The de novo strategy for bifunctional peptides coating to enhance osteointegration capacity of the implant

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-20 DOI:10.1016/j.colsurfb.2025.114642

Siwang Hu , Zeyu Shou , Chengwei Xu , Hongxiang Wang , Zhongyun Li , Xingjie Zan , Na Li , Shihao Xu

{"title":"The de novo strategy for bifunctional peptides coating to enhance osteointegration capacity of the implant","authors":"Siwang Hu , Zeyu Shou , Chengwei Xu , Hongxiang Wang , Zhongyun Li , Xingjie Zan , Na Li , Shihao Xu","doi":"10.1016/j.colsurfb.2025.114642","DOIUrl":null,"url":null,"abstract":"<div><div>Bone implants represent a significant global market; however, they are plagued by a high long-term failure rate, with approximately 19.2 % of implants failing within 10 years. This leads to considerable physical pain, mental distress for patients, and a substantial financial burden on public healthcare systems. Herein, we propose a novel strategy that using the interactions between positively charged hexa-arginine (R6) and polyphenols in EGC/Fe MPN to present the bifunctional peptides, cellular adhesive peptide (RGD) and osteogenic growth peptide (OGP), onto implant coatings. To thoroughly investigate the preparation process and the physical and chemical properties of the dual-peptide functionalized coatings, several techniques were employed, including dissipation-quartz crystal microbalance (DQCM), ellipsometry, photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). These methods provided insights into the coating's composition, stability, mechanical properties, and surface roughness. In comparison to single-peptide functionalized coatings, the dual-peptide coatings demonstrated significantly improved performance in cellular adhesion at early stages, long-term cell proliferation, migration, antioxidant activity, osteogenic differentiation, inhibition of osteoclastogenesis, and enhanced in vivo osteointegration. This study contributes to the development of multifunctional coatings tailored to the complex biological processes involved in osteointegration.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114642"},"PeriodicalIF":5.4000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloids and Surfaces B: Biointerfaces","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0927776525001493","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

Bone implants represent a significant global market; however, they are plagued by a high long-term failure rate, with approximately 19.2 % of implants failing within 10 years. This leads to considerable physical pain, mental distress for patients, and a substantial financial burden on public healthcare systems. Herein, we propose a novel strategy that using the interactions between positively charged hexa-arginine (R6) and polyphenols in EGC/Fe MPN to present the bifunctional peptides, cellular adhesive peptide (RGD) and osteogenic growth peptide (OGP), onto implant coatings. To thoroughly investigate the preparation process and the physical and chemical properties of the dual-peptide functionalized coatings, several techniques were employed, including dissipation-quartz crystal microbalance (DQCM), ellipsometry, photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). These methods provided insights into the coating's composition, stability, mechanical properties, and surface roughness. In comparison to single-peptide functionalized coatings, the dual-peptide coatings demonstrated significantly improved performance in cellular adhesion at early stages, long-term cell proliferation, migration, antioxidant activity, osteogenic differentiation, inhibition of osteoclastogenesis, and enhanced in vivo osteointegration. This study contributes to the development of multifunctional coatings tailored to the complex biological processes involved in osteointegration.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.