Evaluation of the combined impact of aflatoxin B1 and deoxynivalenol fed to Penaeus vannamei and mitigation properties provided by a yeast cell wall extract

Abstract

The purpose of this study was to examine how white shrimp (Penaeus vannamei) responded to varying concentrations of combined mycotoxins (aflatoxin B1 (AFB1) and deoxynivalenol (DON)) as well as the mitigating effects of an adsorbent (yeast cell wall extract, YCWE). The experiment comprised of five groups receiving isonitrogenous and isolipidic diets: CON (control group), LDA (CON with 20 μg/kg AFB1 and 1500 μg/kg DON), HDA (CON with 100 μg/kg AFB1 and 3000 μg/kg DON), LDAM (LDA with 0.2 % YCWE), and HDAM (HDA with 0.2 % YCWE). Results showed that the contents of total protein, total cholesterol, hemocyanin, and enzyme activities (e.g., catalase, phenoloxidase) in plasma were lower in HDA group, while the alanine aminotransferase activity and malondialdehyde content were higher. The HDA group showed up-regulated expression of apoptotic genes in the hepatopancreas but down-regulated expression of immune-related and antioxidant genes. In the LDA and HDA groups, the higher expression of inflammatory genes in hepatopancreas, the lowest chewiness of muscle and the higher abundance of potential pathogens (e.g., Escherichia-Shigella) in the intestine were observed. However, LDAM and HDAM diets were able to enhance the immune response, antioxidant capacity, and hepatopancreatic and intestinal health of shrimp, especially increased the abundance of intestinal bacteria for detoxification (e.g., Pseudomonas). Moreover, dietary YCWE also led to significantly greater mycotoxin content in the feces. In conclusion, the combined mycotoxins AFB1 and DON can negatively affect the health of shrimp, even at lower doses. But adding YCWE to the diets can effectively mitigate these negative effects.