Investigation of the effects of small VCP-interacting protein (SVIP)on the migration and invasion of breast cancer cells

Abstract

Endoplasmic reticulum-associated protein degradation(ERAD) eliminates misfolded proteins. Although there are many proteins involved in ERAD, we focused on small VCP-interacting protein (SVIP). The study aimed to investigate the expression of SVIP in breast cancer types and to determine whether SVIP contributes to the migration and invasion of breast cancer cells. Normal (MCF −10A), estrogen-positive (MCF-7), and triple-negative (MDA-MB-231) breast cancer cell lines were used in this study. Cellular localization of SVIP in breast cancer cells was examined by immunocytochemistry Western blot analysis was used to evaluate protein expression after SVIP siRNA transfection. RTCA identified the consequences of this suppression on cell invasion and migration. The immunoexpression of SVIP was observed in the cytoplasm of MCF-10A, MCF-7, and MDA-MB-231. The transfection of cells with SVIP siRNA led to a reduction in the protein expression of SVIP. SVIPsi RNA suppression results in decreased invasion and migration of MCF −10A. MDA-MB 231 cells' index of invasion and migration increased, and MCF- 7 cell index increased in the invasion but decreased in the migration as a result of SVIP siRNA suppression. In conclusion, SVIPsi transfection of the three cell types altered the ability of migration and invasion of breast cancer cells.