Feeding time modulates the daily rhythms of expression of digestive and metabolic enzymes in the liver, and food intake regulation and reward systems in the hypothalamus of the European sea bass (Dicentrarchus labrax)

Abstract

Fish exhibit daily rhythms at the molecular level across different tissues, synchronized by zeitgebers, such as food availability. To optimize feeding, organisms align internal timekeeping systems to environmental cues. Previous studies on intermediary metabolism and the hypothalamic control of food intake in fish have underscored the significance of feeding time and daily rhythms. This study examined how feeding times—mid-light (ML) versus mid-dark (MD)—influence the rhythmic transcription of digestive and metabolic enzymes in the liver, and regulatory factors of food intake in the hypothalamus of European sea bass (Dicentrarchus labrax). It also explored the connection between food intake control and the reward system. When fish were fed at ML, genes involved in protein digestion (tryp2, tryp3, ctrl, and cpa5) exhibited daily rhythms with peaks early in the dark phase (ZT 11:17–13:36). These peaks were delayed in MD-fed fish (ZT 16:57–18:27). Pla2, a gene related to lipid metabolism, and transamination genes (c-alt, m-alt) showed rhythms only in ML-fed fish, with acrophases in the light phase (ZT 5:01–13:58), such as pyruvate kinase (pk) that peaked at ZT 6:16. Orexigenic genes (npy, orexin) had rhythms only in the MD group, with nocturnal peaks (ZT 13:09, 16:06). Conversely, reward system genes (th, bdnf) were rhythmic in ML-fed fish (ZT 17:35, 11:46), with only th retaining its rhythm in MD-fed fish (ZT 15:30). These findings suggest feeding time significantly affects rhythms in digestive and metabolic processes. They also highlight the intricate nature of food intake regulation systems, which present diverse synchronization patterns in relation to feeding time.