miR-34c-3p Inhibits Nasopharyngeal Carcinoma Development via Inhibiting M2 Polarization of Macrophages.

Yu Zi Ji, Yu Jie Wang, Ji Qing Ma, Zhi Hua Yin, Fei Liu, Yan Zi Zang, Guang Ke Wang, Yong Tai
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Abstract

Objective: miR-34c-3p is down-regulated in nasopharyngeal carcinoma (NPC). The biological role of miR-34c-3p in NPC and its underlying mechanisms are unknown and were explored in this study.

Methods: Flow cytometry and immunohistochemical staining were employed to detect cluster of differentiation 86 (CD86) and cluster of differentiation 206 (CD206) expression; quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were employed to examine mRNA expression and protein levels; cell counting kit-8 (CCK8) and transwell assays were employed to assess cell proliferation, migration, and invasion; and hematoxylin-eosin (HE) staining was employed to assess pathological changes in tumor tissues.

Results: Our results revealed that the miR-34c-3p mimic markedly inhibited M2 polarization of macrophages by targeting SLC7A11, and M2 macrophages transfected with the miR-34c-3p mimic inhibited the proliferation, migration, and invasion of NPC cells. The in vivo experiments further confirmed that miR-34c-3p mimics blocked tumor growth and reduced inflammatory infiltration in tumor tissues.

Conclusion: This study provides novel insights into the pathogenesis of NPC and a new treatment strategy.

miR-34c-3p通过抑制巨噬细胞M2极化抑制鼻咽癌的发展。
目的:miR-34c-3p在鼻咽癌(NPC)中表达下调。miR-34c-3p在鼻咽癌中的生物学作用及其潜在机制尚不清楚,本研究对其进行了探讨。方法:采用流式细胞术和免疫组织化学染色法检测分化簇86 (CD86)和分化簇206 (CD206)的表达;采用实时定量聚合酶链反应(qRT-PCR)和western blotting检测mRNA表达和蛋白水平;采用细胞计数试剂盒-8 (CCK8)和transwell法评估细胞增殖、迁移和侵袭;采用苏木精-伊红(HE)染色评价肿瘤组织病理变化。结果:我们的研究结果显示miR-34c-3p mimic通过靶向SLC7A11显著抑制巨噬细胞的M2极化,转染miR-34c-3p mimic的M2巨噬细胞抑制鼻咽癌细胞的增殖、迁移和侵袭。体内实验进一步证实miR-34c-3p模拟物阻断肿瘤生长,减少肿瘤组织炎症浸润。结论:本研究对鼻咽癌的发病机制和治疗策略提供了新的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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