Mesenchymal stem cell exosomes enhance the development of hair follicle to ameliorate androgenetic alopecia.

IF 3.6 3区医学 Q3 CELL & TISSUE ENGINEERING

World journal of stem cells Pub Date : 2025-03-26 DOI:10.4252/wjsc.v17.i3.102088

Yu Fu, Yao-Ting Han, Jun-Ling Xie, Rong-Qi Liu, Bo Zhao, Xing-Liao Zhang, Jun Zhang, Jing Zhang

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引用次数: 0

Abstract

Background: Mesenchymal stem cells (MSCs) and their secretome have significant potential in promoting hair follicle development. However, the effects of MSC therapy have been reported to vary due to their heterogeneous characteristics. Different sources of MSCs or culture systems may cause heterogeneity of exosomes.

Aim: To define the potential of human adipose-derived MSC exosomes (hADSC-Exos) and human umbilical cord-derived MSC exosomes (hUCMSC-Exos) for improving dermal papillary cell proliferation in androgenetic alopecia.

Methods: We conducted liquid chromatography-mass spectrometry proteomic analysis of hADSC-Exos and hUCMSC-Exos. Liquid chromatography-mass spectrometry suggested that hADSC-Exos were related to metabolism and immunity. Additionally, the hADSC-Exo proteins regulated the cell cycle and other 9 functional groups.

Results: We verified that hADSC-Exos inhibited glycogen synthase kinase-3β expression by activating the Wnt/β-catenin signaling pathway via cell division cycle protein 42, and enhanced dermal papillary cell proliferation and migration. Excess dihydrotestosterone caused androgenetic alopecia by shortening the hair follicle growth phase, but hADSC-Exos reversed these effects.

Conclusion: This study indicated that hair development is influenced by hADSC-Exo-mediated cell-to-cell communication via the Wnt/β-catenin pathway.

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间充质干细胞外泌体促进毛囊发育，改善雄激素性脱发。

背景：间充质干细胞（MSCs）及其分泌组在促进毛囊发育方面具有重要潜力。然而，据报道，骨髓间充质干细胞治疗的效果因其异质性而有所不同。不同来源的间充质干细胞或培养系统可能导致外泌体的异质性。目的：探讨人脂肪源性间充质外泌体（hadscs - exos）和人脐带源性间充质外泌体（hUCMSC-Exos）促进雄激素源性脱发真皮乳头状细胞增殖的潜力。方法：采用液相色谱-质谱法对hdac - exos和hUCMSC-Exos进行蛋白质组学分析。液相色谱-质谱分析表明，hADSC-Exos与代谢和免疫有关。此外，hADSC-Exo蛋白调节细胞周期和其他9个功能基团。结果：我们证实hADSC-Exos通过细胞分裂周期蛋白42激活Wnt/β-catenin信号通路，抑制糖原合酶激酶3β的表达，增强真皮乳头状细胞的增殖和迁移。过量的双氢睾酮通过缩短毛囊生长阶段导致雄激素性脱发，但hADSC-Exos逆转了这些影响。结论：本研究表明hdac - exo介导的Wnt/β-catenin通路对毛发发育有影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World journal of stem cells Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

7.80

自引率

4.90%

发文量

750

期刊介绍： The World Journal of Stem Cells (WJSC) is a leading academic journal devoted to reporting the latest, cutting-edge research progress and findings of basic research and clinical practice in the field of stem cells. It was launched on December 31, 2009 and is published monthly (12 issues annually) by BPG, the world''s leading professional clinical medical journal publishing company.