Study of epidemiological and molecular characteristics of Brucella strains circulating in Kazakhstan.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2025-04-01 DOI:10.1007/s11259-025-10725-9

Aida Daugaliyeva, Saule Daugaliyeva, Aspen Abutalip, Akmaral Adambayeva, Nazerke Kydyr, Simone Peletto

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引用次数: 0

Abstract

Brucellosis is one of the most common zoonotic infectious diseases of animals. The causative agent of brucellosis is a highly conserved bacterium of the genus Brucella, which includes 14 species with minor genetic changes. Our work aimed to study the epidemiological and molecular characteristics of Brucella strains circulating in Kazakhstan, including the study of polymorphisms in critical virulence genes using single nucleotide polymorphism (SNP) analysis based on whole genome sequencing (WGS). A total of 21 Brucella isolates obtained in 2023-2024 from cattle, sheep and goats, horses, camels, pigs, dogs, and humans were analyzed. As a result of whole-genome sequencing, 69 virulence genes were detected. The genome of Brucella melitensis, the most virulent species of Brucella, was the most susceptible to changes. Among the 10 virulence genes studied using SNP, polymorphisms were found in 7 genes. Some genetic mutations resulted in amino acid changes in the coding sequences of these virulence genes. Knowledge of virulence factors and changes in their coding genes has practical implications for epidemiological studies in outbreak areas, their management, and the implementation of brucellosis control strategies.

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哈萨克斯坦布鲁氏菌流行病学及分子特征研究。

布鲁氏菌病是最常见的动物共患传染病之一。布鲁氏菌病的病原体是布鲁氏菌属的一种高度保守的细菌，其中包括14种具有微小遗传变化的细菌。本研究旨在研究哈萨克斯坦布鲁氏菌流行病学和分子特征，包括基于全基因组测序（WGS）的单核苷酸多态性（SNP）分析关键毒力基因多态性。对2023-2024年从牛、绵羊和山羊、马、骆驼、猪、狗和人身上分离的21株布鲁氏菌进行了分析。通过全基因组测序，检测到69个毒力基因。毒力最强的布鲁氏菌（Brucella melitensis）的基因组最容易发生变化。在利用SNP研究的10个毒力基因中，有7个基因存在多态性。一些基因突变导致这些毒力基因编码序列的氨基酸发生变化。了解毒力因子及其编码基因的变化对暴发地区的流行病学研究、管理和实施布鲁氏菌病控制战略具有实际意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.