Strigolactone (GR24) regulates fruit ripening in yft3 tomatoes by altering ABA biosynthesis

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-03-29 DOI:10.1016/j.plantsci.2025.112484

Muhammad Naeem , Rong Han , Anran Xu , Xuemeng Shan , Lingxia Zhao

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引用次数: 0

Abstract

Color development in tomato fruits is a key indicator of ripening, and is driven by complex hormonal and molecular interactions. The present study investigated the effects of exogenous treatment with abscisic acid (ABA), GR24 (a synthetic strigolactone analog), and water (ddH₂O as control) on tomato fruit ripening, ethylene emission, carotenoid biosynthesis, ABA metabolism, and chromoplast development in yft3 and wild-type (WT cv. M82) tomato fruits at 35, 47, and 54 days post-anthesis (dpa). Results showed that GR24 significantly accelerated ripening in yft3, transitioning from green to deep orange at 54 dpa, whereas ABA had a moderate effect. In cv. M82, both treatments enhanced color development, leading to a deep red phenotype. GR24 increased ethylene emission and upregulated the expression of ethylene related genes (ACO1, ACS2/4), with yft3 showing increased sensitivity than cv. M82. Carotenoid profiling revealed higher lycopene and β-carotene contents in yft3, with GR24 enhancing β-carotene and lutein, whereas ABA increased lycopene. GR24 and ABA strongly induced the expression of carotenoid-related genes (CRTISO, PSY1, and CYCB), particularly in yft3 at 54 dpa. Moreover, ABA-content and the expression of genes involved in ABA biosynthesis (NCED, AAO), catabolism (CYP707A), conjugation (GT), and activation (BG) were significantly altered by ABA and GR24 treatments. Furthermore, ultrastructural analysis revealed that GR24 promoted plastoglobule formation and chromoplast differentiation, with yft3 exhibiting a stronger response than cv. M82. These findings highlight that GR24 plays vital roles in regulating tomato fruit ripening, ethylene biosynthesis, carotenoids accumulation, as well as ABA-metabolism, with implications for improving fruit quality in tomatoes.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.