Whole genome analysis and biocontrol potential of endophytic Bacillus cereus EMS1 against Fusarium wilt in banana.

IF 4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

World journal of microbiology & biotechnology Pub Date : 2025-04-01 DOI:10.1007/s11274-025-04326-6

Shilpi Singh, Piyush Kant Rai, Azmat Ali Khan, Sabiha Fatima, Kamlesh Choure, Jeong Chan Joo, Ashutosh Pandey

{"title":"Whole genome analysis and biocontrol potential of endophytic Bacillus cereus EMS1 against Fusarium wilt in banana.","authors":"Shilpi Singh, Piyush Kant Rai, Azmat Ali Khan, Sabiha Fatima, Kamlesh Choure, Jeong Chan Joo, Ashutosh Pandey","doi":"10.1007/s11274-025-04326-6","DOIUrl":null,"url":null,"abstract":"<p><p>Endophytic bacteria are essential for promoting plant growth and increasing plant resilience to various environmental stresses. Although it is well-documented that several endophytic Bacillus species exhibit plant growth-promoting properties, this is the first report on the genome study of Bacillus cereus EMS1, isolated from Musa acuminata G9 in India. This study analyzed the genomics, plant growth traits, and fusarium wilt mitigation potential of Bacillus cereus EMS1. This analysis identified specific genomic features, including potential mechanisms contributing to plant growth promotion, which were also submitted to NCBI (Bioproject ID: PRJNA784269). The in vivo study showed that EMS1 mitigated the impact of Fusarium oxysporum f. sp. cubense on banana plants. Although it did not affect the number of leaves, other parameters influenced by pathogen infection and EMS1 treatment showed notable differences, including fresh weight (Fusarium oxysporum only: 15 g; EMS1 + Fusarium oxysporum: 21 g), dry weight (Fusarium oxysporum only: 1 g; EMS1 + Fusarium oxysporum: 4.7 g), and root length (Fusarium oxysporum only: 6.5 cm; EMS1 + Fusarium oxysporum: 9 cm). Additionally, genomic analysis revealed that the EMS1 genome contains distinctive genes linked to plant growth and antimicrobial activity. Overall, the findings highlight the potential of endophytic Bacillus cereus EMS1 in promoting plant growth and enhancing banana plant resistance against Fusarium oxysporum.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 4","pages":"119"},"PeriodicalIF":4.0000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"World journal of microbiology & biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s11274-025-04326-6","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Endophytic bacteria are essential for promoting plant growth and increasing plant resilience to various environmental stresses. Although it is well-documented that several endophytic Bacillus species exhibit plant growth-promoting properties, this is the first report on the genome study of Bacillus cereus EMS1, isolated from Musa acuminata G9 in India. This study analyzed the genomics, plant growth traits, and fusarium wilt mitigation potential of Bacillus cereus EMS1. This analysis identified specific genomic features, including potential mechanisms contributing to plant growth promotion, which were also submitted to NCBI (Bioproject ID: PRJNA784269). The in vivo study showed that EMS1 mitigated the impact of Fusarium oxysporum f. sp. cubense on banana plants. Although it did not affect the number of leaves, other parameters influenced by pathogen infection and EMS1 treatment showed notable differences, including fresh weight (Fusarium oxysporum only: 15 g; EMS1 + Fusarium oxysporum: 21 g), dry weight (Fusarium oxysporum only: 1 g; EMS1 + Fusarium oxysporum: 4.7 g), and root length (Fusarium oxysporum only: 6.5 cm; EMS1 + Fusarium oxysporum: 9 cm). Additionally, genomic analysis revealed that the EMS1 genome contains distinctive genes linked to plant growth and antimicrobial activity. Overall, the findings highlight the potential of endophytic Bacillus cereus EMS1 in promoting plant growth and enhancing banana plant resistance against Fusarium oxysporum.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物

CiteScore

6.30

自引率

2.40%

发文量

257

审稿时长

2.5 months

期刊介绍： World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.