{"title":"Rolling-phase dynamic full-field OCT.","authors":"Tual Monfort, Kate Grieve, Olivier Thouvenin","doi":"10.1364/OL.543474","DOIUrl":null,"url":null,"abstract":"<p><p>Dynamic full-field optical coherence tomography (DFFOCT) has recently emerged as an invaluable label-free microscopy technique, owing to its sensitivity to cell activity, as well as speed and sectioning ability. However, the quality of DFFOCT images is often degraded due to phase noise and fringe artifacts. In this work, we present a new implementation, to the best of our knowledge, named rolling-phase (RP) DFFOCT, in which the reference arm is slowly scanned over magnitudes exceeding 2<i>π</i>. We demonstrate mathematically and experimentally that it shows superior image quality while enabling to extract both static and dynamic contrast simultaneously. We showcase RP-DFFOCT on a macaque retinal explant and demonstrate its ability to better resolve subcellular structures, including intranuclear activity.</p>","PeriodicalId":19540,"journal":{"name":"Optics letters","volume":"50 7","pages":"2239-2242"},"PeriodicalIF":3.1000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optics letters","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1364/OL.543474","RegionNum":2,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0
Abstract
Dynamic full-field optical coherence tomography (DFFOCT) has recently emerged as an invaluable label-free microscopy technique, owing to its sensitivity to cell activity, as well as speed and sectioning ability. However, the quality of DFFOCT images is often degraded due to phase noise and fringe artifacts. In this work, we present a new implementation, to the best of our knowledge, named rolling-phase (RP) DFFOCT, in which the reference arm is slowly scanned over magnitudes exceeding 2π. We demonstrate mathematically and experimentally that it shows superior image quality while enabling to extract both static and dynamic contrast simultaneously. We showcase RP-DFFOCT on a macaque retinal explant and demonstrate its ability to better resolve subcellular structures, including intranuclear activity.
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The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community.
Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.
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