{"title":"Multicolor 4Pi single-molecule localization based on differences in interference patterns.","authors":"Jianwei Chen, Haoyu Wang, Zhaojun Lin, Yiming Li","doi":"10.1364/OL.553445","DOIUrl":null,"url":null,"abstract":"<p><p>4Pi single-molecule localization microscopy (4Pi-SMLM) achieves sub-10 nm isotropic three-dimensional resolution, representing a significant advancement in super-resolution imaging. However, traditional multicolor imaging techniques generally require additional modifications to the optical path, which complicates the system and leads to photon loss. In this study, we introduce a new, to the best of our knowledge, multicolor imaging method that leverages the differences in 4Pi-PSF size and interference fringe spacing caused by wavelength variations, enabling multicolor 4Pi-SMLM imaging without hardware modifications. This method captures color information directly from the raw data by utilizing wavelength-dependent point spread functions (PSFs). By globally fitting the data within multiple interference channels, we could achieve both high localization accuracy and color separation accuracy for single molecules of different colors.</p>","PeriodicalId":19540,"journal":{"name":"Optics letters","volume":"50 7","pages":"2346-2349"},"PeriodicalIF":3.1000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optics letters","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1364/OL.553445","RegionNum":2,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0
Abstract
4Pi single-molecule localization microscopy (4Pi-SMLM) achieves sub-10 nm isotropic three-dimensional resolution, representing a significant advancement in super-resolution imaging. However, traditional multicolor imaging techniques generally require additional modifications to the optical path, which complicates the system and leads to photon loss. In this study, we introduce a new, to the best of our knowledge, multicolor imaging method that leverages the differences in 4Pi-PSF size and interference fringe spacing caused by wavelength variations, enabling multicolor 4Pi-SMLM imaging without hardware modifications. This method captures color information directly from the raw data by utilizing wavelength-dependent point spread functions (PSFs). By globally fitting the data within multiple interference channels, we could achieve both high localization accuracy and color separation accuracy for single molecules of different colors.
期刊介绍：
The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community.
Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.
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