Isotropic spectrum optimization for enhancing image fidelity in super-resolution structured illumination microscopy.

IF 3.1 2区物理与天体物理 Q2 OPTICS

Optics letters Pub Date : 2025-04-01 DOI:10.1364/OL.558342

Yong Hu, Ge Wu, Zhi Chen, Tao Yang, Liao Liang, Ziqiang Tu, Xiaolu Ni, Rong Wan, Qiuqiang Zhan, Zewei Luo, Tongsheng Chen

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引用次数: 0

Abstract

Super-resolution structured illumination microscopy (SR-SIM) performs spectral expansion of high-frequency information encoded in stripe patterns. However, using a limited number of pattern orientations (typically three) results in a petal-like frequency spectrum, leading to structural and intensity fidelity degradation in reconstructed images. In this Letter, we propose an integrated spatial-frequency domain SIM reconstruction method that enables isotropic spectrum expansion, called ISO-SIM. ISO-SIM overcomes structural artifacts caused by an anisotropic spectral expansion in traditional SIM imaging. We demonstrate the feasibility and fidelity of ISO-SIM through simulations, Argolight slide, and live-cell imaging. ISO-SIM enhanced structural similarity and reduced the error in the mean intensity ratio at certain spatial frequencies compared to Wiener-SIM. We further applied ISO-SIM to live-cell quantitative FRET imaging. ISO-SIM-FRET ensured that the measured FRET efficiency matched the ground truth, with a 19% reduction in the standard deviation compared to Wiener-SIM-FRET, maintaining intensity fidelity and enhancing the accuracy of quantitative analysis while suppressing artifacts.

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提高超分辨率结构照明显微镜图像保真度的各向同性光谱优化。

超分辨率结构照明显微镜（SR-SIM）对编码在条纹图案中的高频信息进行光谱扩展。然而，使用有限数量的模式取向（通常是三个）会导致花瓣状频谱，导致重建图像的结构和强度保真度下降。在这篇文章中，我们提出了一种集成的空间频域SIM重建方法，使各向同性频谱扩展，称为ISO-SIM。ISO-SIM克服了传统SIM成像中各向异性光谱展开引起的结构伪影。我们通过模拟，Argolight幻灯片和活细胞成像证明了ISO-SIM的可行性和保真度。与Wiener-SIM相比，ISO-SIM增强了结构相似性，减小了特定空间频率下的平均强度比误差。我们进一步将ISO-SIM应用于活细胞定量FRET成像。ISO-SIM-FRET确保测量的FRET效率与地面真实相匹配，与Wiener-SIM-FRET相比，标准偏差降低19%，保持强度保真度，提高定量分析的准确性，同时抑制伪影。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Optics letters 物理-光学

CiteScore

6.60

自引率

8.30%

发文量

2275

审稿时长

1.7 months

期刊介绍： The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community. Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.