Isotropic spectrum optimization for enhancing image fidelity in super-resolution structured illumination microscopy.

IF 3.1 2区物理与天体物理 Q2 OPTICS

Optics letters Pub Date : 2025-04-01 DOI:10.1364/OL.558342

Yong Hu, Ge Wu, Zhi Chen, Tao Yang, Liao Liang, Ziqiang Tu, Xiaolu Ni, Rong Wan, Qiuqiang Zhan, Zewei Luo, Tongsheng Chen

{"title":"Isotropic spectrum optimization for enhancing image fidelity in super-resolution structured illumination microscopy.","authors":"Yong Hu, Ge Wu, Zhi Chen, Tao Yang, Liao Liang, Ziqiang Tu, Xiaolu Ni, Rong Wan, Qiuqiang Zhan, Zewei Luo, Tongsheng Chen","doi":"10.1364/OL.558342","DOIUrl":null,"url":null,"abstract":"<p><p>Super-resolution structured illumination microscopy (SR-SIM) performs spectral expansion of high-frequency information encoded in stripe patterns. However, using a limited number of pattern orientations (typically three) results in a petal-like frequency spectrum, leading to structural and intensity fidelity degradation in reconstructed images. In this Letter, we propose an integrated spatial-frequency domain SIM reconstruction method that enables isotropic spectrum expansion, called ISO-SIM. ISO-SIM overcomes structural artifacts caused by an anisotropic spectral expansion in traditional SIM imaging. We demonstrate the feasibility and fidelity of ISO-SIM through simulations, Argolight slide, and live-cell imaging. ISO-SIM enhanced structural similarity and reduced the error in the mean intensity ratio at certain spatial frequencies compared to Wiener-SIM. We further applied ISO-SIM to live-cell quantitative FRET imaging. ISO-SIM-FRET ensured that the measured FRET efficiency matched the ground truth, with a 19% reduction in the standard deviation compared to Wiener-SIM-FRET, maintaining intensity fidelity and enhancing the accuracy of quantitative analysis while suppressing artifacts.</p>","PeriodicalId":19540,"journal":{"name":"Optics letters","volume":"50 7","pages":"2461-2464"},"PeriodicalIF":3.1000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optics letters","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1364/OL.558342","RegionNum":2,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPTICS","Score":null,"Total":0}

引用次数: 0

Abstract

Super-resolution structured illumination microscopy (SR-SIM) performs spectral expansion of high-frequency information encoded in stripe patterns. However, using a limited number of pattern orientations (typically three) results in a petal-like frequency spectrum, leading to structural and intensity fidelity degradation in reconstructed images. In this Letter, we propose an integrated spatial-frequency domain SIM reconstruction method that enables isotropic spectrum expansion, called ISO-SIM. ISO-SIM overcomes structural artifacts caused by an anisotropic spectral expansion in traditional SIM imaging. We demonstrate the feasibility and fidelity of ISO-SIM through simulations, Argolight slide, and live-cell imaging. ISO-SIM enhanced structural similarity and reduced the error in the mean intensity ratio at certain spatial frequencies compared to Wiener-SIM. We further applied ISO-SIM to live-cell quantitative FRET imaging. ISO-SIM-FRET ensured that the measured FRET efficiency matched the ground truth, with a 19% reduction in the standard deviation compared to Wiener-SIM-FRET, maintaining intensity fidelity and enhancing the accuracy of quantitative analysis while suppressing artifacts.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Optics letters 物理-光学

CiteScore

6.60

自引率

8.30%

发文量

2275

审稿时长

1.7 months

期刊介绍： The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community. Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.