Mannheimia granulomatis-Infected Pyogranulomatous Oral Lesions with Concurrent Amyloidosis in Roe Deer (Capreolus capreolus) in France.

IF 1.1 4区 农林科学 Q3 VETERINARY SCIENCES
Rozenn Le Net, Benoît Thuillier, Pascal Bihannic, Jessica Schmitz, Jan H Bräsen, Reinhold P Linke, Anouk Decors, Karin Lemberger
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引用次数: 0

Abstract

Abstract: Mannheimia granulomatis-associated lesions have been poorly described compared with those associated with other members of the Pasteurellaceae family; they include pneumonia, panniculitis, and keratoconjunctivitis in domestic and wild ruminants and hares. We describe 15 cases of roe deer (Capreolus capreolus) showing M. granulomatis-infected oral lesions that were detected in France between 2016 and 2023. Comprehensive necropsies were performed, with additional analyses based on findings. All deer were >15 mo of age on the basis of tooth eruption. They were thin (1/15), cachectic (13/15), or of unknown body condition (1/15). They exhibited multiple cryptic millimetric to centimetric caseous granulomas within the lips, tongue, or cheeks (13/15); pyogranulomatous lymphadenitis of a mandibular lymph node (1/15); or necrosuppurative mandibular osteomyelitis with bone proliferation (1/15). Histopathologically, oral lesions of 10/15 animals showed multifocal, severe, chronic pyogranulomatous cheilitis, glossitis, or stomatitis with intralesional Gram-negative bacterial colonies lined by Splendore-Hoeppli material. Amyloidosis was detected in 4/11 animals for which tissues were submitted for microscopic examination, affecting the abomasum, the spleen, and a minor salivary gland in, respectively, 4/6, 3/5, and 1/8 animals for which the relevant organs were assessed histologically. Amyloid deposits showed strong immunoreactivity with a murine monoclonal antibody directed against amyloid AA. AA amyloidosis, never previously described in roe deer, was considered secondary to chronic inflammation. Mannheimia granulomatis was cultured and identified biochemically or by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in oral lesions of all individuals. Coinfection with other bacterial species was sporadically identified. We considered the chronic emaciation and death of these animals to be multifactorial, involving oral lesions, secondary amyloidosis when present, parasitic infestation of variable severity, and probably environmental factors. Further investigation is needed to understand the epidemiology and pathogenesis of M. granulomatis infection in roe deer, including a possible propensity to emerge as temporal and geographic clusters and to induce AA amyloidosis.

法国鹿(Capreolus Capreolus)肉芽肿感染的脓肉芽肿性口腔病变并发淀粉样变性。
摘要:与巴氏杆菌科其他成员的相关病变相比,mannheia肉芽肿相关病变的描述很少;它们包括家养和野生反刍动物和野兔的肺炎、膜炎和角膜结膜炎。我们描述了2016年至2023年在法国检测到的15例鹿(Capreolus Capreolus)口腔病变,显示肉芽肿分枝杆菌感染。进行了全面的尸检,并根据发现进行了额外的分析。所有的鹿都在15月龄左右。他们瘦(1/15),恶病质(13/15),或身体状况不明(1/15)。他们在嘴唇、舌头或脸颊内表现出多个隐匿的毫米至厘米级干酪样肉芽肿(13/15);下颌骨淋巴结脓肉芽肿性淋巴结炎(1/15);或坏死化脓性下颌骨髓炎伴骨增生(1/15)。在组织病理学上,10/15只动物的口腔病变表现为多灶性、严重的慢性脓肉芽肿性口炎、舌炎或口炎,病灶内革兰氏阴性菌落排列有Splendore-Hoeppli物质。在4/11的动物组织镜检中检测到淀粉样变,分别在4/6、3/5和1/8的动物中检测到皱胃、脾脏和小唾液腺。淀粉样蛋白沉积物对一种针对淀粉样蛋白AA的小鼠单克隆抗体显示出很强的免疫反应性。AA淀粉样变,以前从未在狍中描述过,被认为是继发于慢性炎症。在所有个体的口腔病变中培养并通过生化或基质辅助激光解吸/电离飞行时间质谱法鉴定了曼海姆肉芽肿。偶有发现与其他细菌共感染。我们认为这些动物的慢性消瘦和死亡是多因素的,包括口腔病变、继发性淀粉样变、不同严重程度的寄生虫感染,以及可能的环境因素。需要进一步研究鹿肉芽肿支原体感染的流行病学和发病机制,包括可能出现的时间和地理聚集性和诱导AA淀粉样变的倾向。
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来源期刊
Journal of Wildlife Diseases
Journal of Wildlife Diseases 农林科学-兽医学
CiteScore
2.70
自引率
0.00%
发文量
213
审稿时长
6-16 weeks
期刊介绍: The JWD publishes reports of wildlife disease investigations, research papers, brief research notes, case and epizootic reports, review articles, and book reviews. The JWD publishes the results of original research and observations dealing with all aspects of infectious, parasitic, toxic, nutritional, physiologic, developmental and neoplastic diseases, environmental contamination, and other factors impinging on the health and survival of free-living or occasionally captive populations of wild animals, including fish, amphibians, reptiles, birds, and mammals. Papers on zoonoses involving wildlife and on chemical immobilization of wild animals are also published. Manuscripts dealing with surveys and case reports may be published in the Journal provided that they contain significant new information or have significance for better understanding health and disease in wild populations. Authors are encouraged to address the wildlife management implications of their studies, where appropriate.
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