{"title":"Exploring Anti-Aging Potential of <i>Dendrobium</i> Species and Novel Microemulsion Delivery of <i>Dendrobium kentrophyllum</i> Extract for Anti-Aging Effect.","authors":"Suradwadee Thungmungmee, Boonchoo Sritularak, Nakuntwalai Wisidsri, Nattakan Kanana, Nattika Nimmano","doi":"10.2147/NSA.S508582","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The study aimed to investigate in vitro anti-aging activities of 29 <i>Dendrobium</i> spp. and develop and characterize microemulsions (MEs) for topical application.</p><p><strong>Methods: </strong>Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), H<sub>2</sub>O<sub>2</sub> scavenging, and ferric reducing antioxidant power (FRAP) assays. The anti-collagenase (MMP-1 and MMP-2) and anti-elastase activities were also evaluated. Cytotoxicity and human intracellular reactive oxygen species (ROS) levels were determined using resazurin reduction and 2',7'-dichlorofluorescin diacetate (DCFDA) assays, respectively. <i>D. kentrophyllum</i> extract-loaded microemulsion (DKME) was then prepared and optimized. The stability of DKME was studied using a heating-cooling cycle.</p><p><strong>Results: </strong><i>D. kentrophyllum</i> appeared to be the best candidate anti-aging agent because of its antioxidant, anti-collagenase, and anti-elastase activities. The extract was safe for human skin cells at a concentration of 6.25-100 μg/mL. It also decreased the intracellular ROS-induced ultraviolet B (UVB) irradiation compared to that in the control. DKME comprising Tween 80:ethanol (5:1), water, and isononyl isononanoate showed a suitable appearance, droplet size, polydisperse index, zeta potential, pH, and viscosity. This formulation demonstrated desirable physical and chemical stability, with non-cytotoxic effects.</p><p><strong>Conclusion: </strong>DKME is considered a promising anti-aging product. However, an in vivo study of this optimized formulation might be evaluated in further study for anti-aging purposes.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"157-171"},"PeriodicalIF":4.9000,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11951923/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanotechnology, Science and Applications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2147/NSA.S508582","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: The study aimed to investigate in vitro anti-aging activities of 29 Dendrobium spp. and develop and characterize microemulsions (MEs) for topical application.
Methods: Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), H2O2 scavenging, and ferric reducing antioxidant power (FRAP) assays. The anti-collagenase (MMP-1 and MMP-2) and anti-elastase activities were also evaluated. Cytotoxicity and human intracellular reactive oxygen species (ROS) levels were determined using resazurin reduction and 2',7'-dichlorofluorescin diacetate (DCFDA) assays, respectively. D. kentrophyllum extract-loaded microemulsion (DKME) was then prepared and optimized. The stability of DKME was studied using a heating-cooling cycle.
Results: D. kentrophyllum appeared to be the best candidate anti-aging agent because of its antioxidant, anti-collagenase, and anti-elastase activities. The extract was safe for human skin cells at a concentration of 6.25-100 μg/mL. It also decreased the intracellular ROS-induced ultraviolet B (UVB) irradiation compared to that in the control. DKME comprising Tween 80:ethanol (5:1), water, and isononyl isononanoate showed a suitable appearance, droplet size, polydisperse index, zeta potential, pH, and viscosity. This formulation demonstrated desirable physical and chemical stability, with non-cytotoxic effects.
Conclusion: DKME is considered a promising anti-aging product. However, an in vivo study of this optimized formulation might be evaluated in further study for anti-aging purposes.
期刊介绍：
Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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