Transcriptome Response of Rosa roxburghii Fruit to Top-Rot Disease and Functional Analysis of RrHSP18.1 in Top-Rot Disease Resistance.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES

Phytopathology Pub Date : 2025-03-31 DOI:10.1094/PHYTO-01-25-0015-R

Minggui Li, Min Lu, Xiaomao Wu, Huaming An

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引用次数: 0

Abstract

Top-rot disease (TRD) in Rosa roxburghii fruit is caused by Colletotrichum fructicola. TRD has emerged as a significant concern due to its frequent occurrence and cause of substantial economic losses. To understand the transcriptome response to TRD infection and identify candidate genes involved in TRD resistance, four critical time points (0, 24, 72, and 120 hpi) fruit tissues inoculated with TRD pathogen were selected for RNA-seq. A total of 1,890 differentially expressed genes (DEGs) were identified from the transcriptome data, including 1,051 upregulated and 839 downregulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that they were primarily involved in oxidoreductase activity and the synthesis and metabolism secondary metabolites. Many putative transcription factor families such as MYB, bHLH, AP2-EREBP, NAC, and WRKY were also identified as being positively responsive to TRD infestation. Among the upregulated genes, RrHSP18.1 exhibited the strongest response to TRD. Transient overexpression and gene silencing demonstrated that RrHSP18.1 positively regulated TRD resistance in R. roxburghii fruit, partially through promoting the expression of antioxidant-related genes and enhancing their enzyme activities. Collectively, the results facilitated a better understanding of the transcriptional response to TRD and offered candidate genes for developing R. roxburghii germplasm resource with improved TRD resistance.

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来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.