Curcumin-gold nanoshell mediated near-infrared irradiation on human ovarian cancer cell: in vitro study.

Abstract

Ovarian cancer is considered a predominant female reproductive malignancy and poses a significant threat due to its 80-90% fatality rate. The typical approach involves surgery and chemotherapy, which due to problems such as drug resistance, encourage researchers to use new methods such as nanotechnology. The current study introduces a novel strategy: leveraging Curcumin-Gold Nanoshells (Cur-AuNShs) to combat chemotherapy's adverse effects and overcome drug resistance through hyperthermia mediation. Gold-based nanoparticles that absorb laser have shown the potential to target and treat cancer selectively through highly efficient light-to-heat conversion. This experimental study focused on the synthesis of AuNShs and their subsequent conjugation with Cur. The gold shell coverage on the surfaces of silica nanoparticles was examined using UV-VIS spectroscopy and transmission electron microscopy (TEM). Dynamic light scattering (DLS) and Zeta potential analysis were employed to evaluate the stability of particle size and surface charge. Human ovarian carcinoma cell lines (SKOV-3) were treated with a combination of Cur (15 μM) and AuNShs (75 μM), under the activation of near-infrared (NIR) laser irradiation at a power of 2.5 W/cm³ for 5 or 10 min. Cell viability was then assessed using the MTT assay. Lastly, the expression levels of Bax, Bcl2, and HSPB1 genes were analyzed using the real-time polymerase chain reaction (real-time PCR) technique. The average diameter of the AuNShs was measured at 70 ± 7.1 nm. Findings revealed that after a 48 h incubation with Cur-AuNShs followed by 10 min of laser irradiation, cell viability decreased significantly from 44.3 ± 1.7 to 14.4 ± 1. Analysis using real-time PCR showed an increase in Bax expression alongside a decrease in Bcl2 expression. Additionally, the expression of the HSPB1 gene was reduced from 1.35 ± 1 to 0.9 ± 0.65 in the laser-treated Cur-AuNShs-NIR group. The AuNShs, when combined with hyperthermia at 43 °C, demonstrated potential as an effective carrier for Cur administration. This combination was associated with a greater activation of apoptosis compared to the free drug.