Ameliorating Effect of Red Fruit (Pandanus conoides Lamk) Oil in Streptozotosin-Induced Diabetic Peripheral Neuropathy Rat Model: role of oxidative and neuroinflammatory pathways.
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引用次数: 0
Abstract
Objectives: Diabetic peripheral neuropathy (DPN) is the most common cause of neuropathy worldwide, with oxidative and inflammatory being the pathways involved in the disease pathology. Red fruit (Pandanus conoides Lamk) oil (RFO) is known to have potent antioxidants and anti-inflammatory activities. To investigate the effects of RFO in a rat model of streptozotocin-induced DPN.
Methods: Male Wistar rats were induced with 55 mg/kg streptozotocin (STZ) intraperitoneally. Rats with fasting blood glucose ≥ 200 mg/dL were assigned into groups treated with vehicle, pregabalin 30 mg/kg, and 0.3, 0.6, or 1.2 mL/kg RFO, respectively. The treatments were given for 3 weeks, six weeks after STZ induction. Neuropathic pain was evaluated by cold allodynia and thermal hyperalgesia tests. Histopathological changes of the pancreas and sciatic nerve were evaluated by the Gaussian adaptive threshold method. Feed and drinking intake, blood glucose levels, body weight, and sciatic nerve proinflammatory cytokines were also measured.
Results: There were increases in blood glucose levels, feed and drink intake, levels of MDA, TNF-α, IL-6, NFκB, and iNOS; decrease in catalase level; and damage to the sciatic nerve in the vehicle-treated DPN rat model compared with normal rats (p < 0.05). Meanwhile, significant decrease in weight, number of pancreatic β cells, latency times for thermal hyperalgesia as well as cold allodynia were observed in this group of rats. RFO administration at 0.6 and 1.2 mL/kg significantly improved all of the measured indices, except for iNOS, where only decreasing trend was observed. Pregabalin did not affect blood glucose levels, body weight or pancreatic β cells but improved thermal hyperalgesia as well as cold allodynia, the sciatic nerve cell counts, TNF-α IL-6, NFκB, and iNOS levels.
Conclusion: RFO improves DPN by neuroprotective effect through the involvement of the oxidative and neuroinflammatory pathways.
期刊介绍：
The Journal of Pharmacopuncture covers a wide range of basic and clinical science research relevant to all aspects of the biotechnology of integrated approaches using both pharmacology and acupuncture therapeutics, including research involving pharmacology, acupuncture studies and pharmacopuncture studies. The subjects are mainly divided into three categories: pharmacology (applied phytomedicine, plant sciences, pharmacology, toxicology, medicinal plants, traditional medicines, herbal medicine, Sasang constitutional medicine, herbal formulae, foods, agricultural technologies, naturopathy, etc.), acupuncture (acupressure, electroacupuncture, laser acupuncture, moxibustion, cupping, etc.), and pharmacopuncture (aqua-acupuncture, meridian pharmacopuncture, eight-principles pharmacopuncture, animal-based pharmacopuncture, mountain ginseng pharmacopuncture, bee venom therapy, needle embedding therapy, implant therapy, etc.). Other categories include chuna treatment, veterinary acupuncture and related animal studies, alternative medicines for treating cancer and cancer-related symptoms, etc. Broader topical coverage on the effects of acupuncture, the medical plants used in traditional and alternative medicine, pharmacological action and other related modalities, such as anthroposophy, homeopathy, ayurveda, bioelectromagnetic therapy, chiropractic, neural therapy and meditation, can be considered to be within the journal’s scope if based on acupoints and meridians. Submissions of original articles, review articles, systematic reviews, case reports, brief reports, opinions, commentaries, medical lectures, letters to the editor, photo-essays, technical notes, and book reviews are encouraged. Providing free access to the full text of all current and archived articles on its website (www.journal.ac), also searchable through a Google Scholar search.
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