microRNA miR-315-5p regulates developmental growth in Drosophila wings by targeting S6k.

IF 2.9 1区 农林科学 Q1 ENTOMOLOGY
Taeheon Lee, Chae Jeong Kim, Do-Hwan Lim, Young Sik Lee
{"title":"microRNA miR-315-5p regulates developmental growth in Drosophila wings by targeting S6k.","authors":"Taeheon Lee, Chae Jeong Kim, Do-Hwan Lim, Young Sik Lee","doi":"10.1111/1744-7917.70027","DOIUrl":null,"url":null,"abstract":"<p><p>Tissue growth in Drosophila is regulated by various factors, with microRNAs (miRNAs) emerging as key players over the past decade. However, the precise roles of miRNAs in growth regulation remain incompletely understood. In this study, we explored the biological role of miR-315 in wing growth regulation. Inhibition of miR-315-5p activity using a miR-315 sponge led to an increase in wing size, whereas its overexpression resulted in reduced wing size, primarily through a decrease in wing cell size. We identified ribosomal protein kinase p-70-S6k (S6k) as a target of miR-315-5p in relation to wing growth control. Overexpression of miR-315 reduced both total S6k and phosphorylated S6k protein levels in Drosophila S2 cells and wing discs. Additionally, a luciferase reporter assay confirmed that miR-315-5p directly binds to the 3'-untranslated region of S6k. Consistently, RNAi-mediated depletion of S6k led to smaller wings, primarily due to a reduction in cell size. Notably, co-overexpression of active S6k rescued the wing defects caused by miR-315 overexpression. Overall, these findings demonstrate that miR-315 regulates wing growth by suppressing S6k expression.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/1744-7917.70027","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Tissue growth in Drosophila is regulated by various factors, with microRNAs (miRNAs) emerging as key players over the past decade. However, the precise roles of miRNAs in growth regulation remain incompletely understood. In this study, we explored the biological role of miR-315 in wing growth regulation. Inhibition of miR-315-5p activity using a miR-315 sponge led to an increase in wing size, whereas its overexpression resulted in reduced wing size, primarily through a decrease in wing cell size. We identified ribosomal protein kinase p-70-S6k (S6k) as a target of miR-315-5p in relation to wing growth control. Overexpression of miR-315 reduced both total S6k and phosphorylated S6k protein levels in Drosophila S2 cells and wing discs. Additionally, a luciferase reporter assay confirmed that miR-315-5p directly binds to the 3'-untranslated region of S6k. Consistently, RNAi-mediated depletion of S6k led to smaller wings, primarily due to a reduction in cell size. Notably, co-overexpression of active S6k rescued the wing defects caused by miR-315 overexpression. Overall, these findings demonstrate that miR-315 regulates wing growth by suppressing S6k expression.

求助全文
约1分钟内获得全文 求助全文
来源期刊
Insect Science
Insect Science 生物-昆虫学
CiteScore
7.80
自引率
5.00%
发文量
1379
审稿时长
6.0 months
期刊介绍: Insect Science is an English-language journal, which publishes original research articles dealing with all fields of research in into insects and other terrestrial arthropods. Papers in any of the following fields will be considered: ecology, behavior, biogeography, physiology, biochemistry, sociobiology, phylogeny, pest management, and exotic incursions. The emphasis of the journal is on the adaptation and evolutionary biology of insects from the molecular to the ecosystem level. Reviews, mini reviews and letters to the editor, book reviews, and information about academic activities of the society are also published.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信