Evaluation of the NG-Test Carba 5 for the clinical detection of carbapenemase-producing gram-negative bacteria.

IF 3.1 3区医学 Q1 MEDICINE, GENERAL & INTERNAL

Frontiers in Medicine Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI:10.3389/fmed.2025.1512345

Hai-Feng Qin, Jin-Ke He, Xin Chen, Ke Jiang, Xiao-Yan Cai, Xiao-Ni Wu, Lei Ye, Hao-Kai Chen, Xu-Guang Guo, Yong Xia

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引用次数: 0

Abstract

Background: Currently, the spread and prevalence of carbapenem-resistant gram-negative bacteria cause a worldwide health problem, significantly affecting patients' prognosis. Therefore, reliable detection of carbapenemases is crucial for managing and controlling infections. Numerous investigations have shown that the innovative immunochromatographic assay NG-Test Carba 5 has great sensitivity and specificity for carbapenemase typing. This meta-analysis aimed to comprehensively assess the efficacy of the NG-Test Carba 5 in the clinical detection of carbapenemase-producing gram-negative bacteria.

Methods: Previously published articles were systematically reviewed, relevant data were extracted, and the results were pooled and analyzed using Meta-DiSk 1.4 and Stata 12.0 software.

Results: The sensitivity, specificity, positive LR value, and negative LR value for the identification of carbapenemase-type KPC, NDM, VIM, IMP, and OXA-48-like by immunochromatographic NG-Test Carba 5 using PCR as gold standard were 0.97 [95% CI (0.97, 0.98)], 0.99 [95% CI (0.99, 1.00)], 65.38 [95% CI (36.73, 116.39)], and 0.03 [95% CI (0.02, 0.05)], respectively, and the combined diagnostic odds ratio was 2,734.42 [95% CI (1,464.05, 5,107.12)]. The AUC of the SROC curve was 0.9976.

Conclusion: In summary, the NG-Test Carba 5 is a clinical test that can identify and quickly detect five major carbapenemases, thus offering valuable insights for clinical decision-making and infection control.

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来源期刊

Frontiers in Medicine Medicine-General Medicine

CiteScore

5.10

自引率

5.10%

发文量

3710

审稿时长

12 weeks

期刊介绍： Frontiers in Medicine publishes rigorously peer-reviewed research linking basic research to clinical practice and patient care, as well as translating scientific advances into new therapies and diagnostic tools. Led by an outstanding Editorial Board of international experts, this multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. In addition to papers that provide a link between basic research and clinical practice, a particular emphasis is given to studies that are directly relevant to patient care. In this spirit, the journal publishes the latest research results and medical knowledge that facilitate the translation of scientific advances into new therapies or diagnostic tools. The full listing of the Specialty Sections represented by Frontiers in Medicine is as listed below. As well as the established medical disciplines, Frontiers in Medicine is launching new sections that together will facilitate - the use of patient-reported outcomes under real world conditions - the exploitation of big data and the use of novel information and communication tools in the assessment of new medicines - the scientific bases for guidelines and decisions from regulatory authorities - access to medicinal products and medical devices worldwide - addressing the grand health challenges around the world