Metabolomics Analysis of the Mechanism of Yuquan Capsules in the Treatment of T2DM Rats Using UPLC/MS.

IF 2.8 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy Pub Date : 2025-03-27 eCollection Date: 2025-01-01 DOI:10.2147/DMSO.S512089

Mengli Zhang, Zhien Tan, Min Ou, Taiping Li, Xiaonan Yang, Lu Chen, Rui Xiao, Na Lu, Xijun Wang, Fangfang Wu

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Abstract

Purpose: Yuquan capsule (YQC) is a well-known proprietary Chinese medicine used for the treatment of type 2 diabetes mellitus. The aim of this study was to investigate the potential mechanism and efficacy of YQC in the treatment of T2DM by means of metabolomics.

Methods: Thirty-two male SD rats were randomly divided into four groups of control, type 2 diabetic mellitus (T2DM), metformin (Met), and YQC. Establishment of the T2DM model by high-fat diet (HFD) and streptozotocin (STZ). Fasting blood glucose (FBG) and weight were measured weekly, urine output was collected and recorded. The blood, kidney, pancreas, and liver tissue samples were collected at the end of the experiment. Blood samples were analyzed with methods of ELISA, pancreas, and liver tissues were analyzed by pathological sections, and serum was analyzed by metabolomics using ultra-performance liquid chromatography quadrupole time-of-flight coupled with mass spectrometry (UPLC-Q/TOF-MS).

Results: It was observed that YQC could reduce blood glucose levels by modulating blood lipid and transaminase indices, and by diminishing the concentration of inflammatory factors within hepatic and pancreatic tissues. Furthermore, YQC restores homeostasis by regulating lipid and amino acid metabolism, engaging 21 biomarkers and 10 metabolic pathways.

Conclusion: YQC has the capacity to enhance blood lipid and transaminase levels, suppress the expression of inflammatory factors, and foster the homeostatic progression of metabolic circulation in rats with T2DM.

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UPLC/MS分析玉泉胶囊治疗2型糖尿病大鼠的代谢组学机制

目的：玉泉胶囊（YQC）是一种治疗2型糖尿病的知名中成药。本研究旨在通过代谢组学方法研究玉泉胶囊治疗 T2DM 的潜在机制和疗效：32只雄性SD大鼠被随机分为对照组、2型糖尿病组（T2DM）、二甲双胍组（Met）和YQC组四组。通过高脂饮食（HFD）和链脲佐菌素（STZ）建立 T2DM 模型。每周测量空腹血糖（FBG）和体重，收集并记录尿量。实验结束时收集血液、肾脏、胰腺和肝脏组织样本。血样用酶联免疫吸附法进行分析，胰腺和肝脏组织用病理切片法进行分析，血清用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q/TOF-MS）进行代谢组学分析：结果表明：YQC 可通过调节血脂和转氨酶指数以及降低肝脏和胰腺组织内炎症因子的浓度来降低血糖水平。此外，YQC 还能通过调节脂质和氨基酸代谢、参与 21 个生物标志物和 10 条代谢途径来恢复体内平衡：结论：YQC 能够提高 T2DM 大鼠的血脂和转氨酶水平，抑制炎症因子的表达，促进代谢循环的平衡进展。

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来源期刊

Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

5.90

自引率

6.10%

发文量

431

审稿时长

16 weeks

期刊介绍： An international, peer-reviewed, open access, online journal. The journal is committed to the rapid publication of the latest laboratory and clinical findings in the fields of diabetes, metabolic syndrome and obesity research. Original research, review, case reports, hypothesis formation, expert opinion and commentaries are all considered for publication.