The effects of alumina and polystyrene nanoparticles on global DNA methylation, antimicrobial peptides and intergenerational inheritance of Galleria mellonella.
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引用次数: 0
Abstract
The epigenetic and immunological effects of nanoparticles (NPs), which have started to be described as nano-pollutants today, are of great interest in living organisms. Particularly alumina (Al) and polystyrene (PS) are among the most produced NPs. Galleria mellonella larvae, an ideal model for the multi-generational effects of these NPs on global DNA methylation and the immune system, were used in the experiments. Al-NPs were bought, and PS-NPs were produced by the single emulsion solvent evaporation method. Al and PS-NPs were administered to larvae at different concentrations by changing only the water content in the diet. Global DNA methylation levels in the first and second generations were determined by HPLC. The expression levels of β-actin, transferrin, galiomycin, and p38 MAPK genes which constitute antimicrobial peptides, one of the humoral immune responses, were determined by RT-qPCR in two generations. The data obtained revealed that Al and PS-NPs increased global DNA methylation, and partially suppressed humoral immune responses. Furthermore, changes in genomic DNA methylation and immune-related gene expression levels induced by NPs in first generation larvae were found to be inherited by the next generation. Considering the importance of multigenerational epigenetic effects and changes in the immune system, our study results contribute to the literature and reveal the importance of such studies.
期刊介绍：
Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal.
Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.
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