Efficient procedure for human adipose tissue cryopreservation without specialized freezing equipment.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2025-05-01

B Heidari, H Jafary, H Golshahi, H Soltanghoraei, S Shams, A Soltani, S M Tabaie

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引用次数: 0

Abstract

Background: Adipose tissue grafting is one of the reconstruction methods for damaged tissue repair.

Objective: To develop a convenient procedure for human adipose tissue cryopreservation without any special equipment.

Materials and methods: Adipose tissues were frozen using different combinations of permeating and non-permeating cryoprotectants at a cooling rate of -1 degree C per min and stored at -20 degree C for 1, 3, 6 and 9 months. Histo-morphological characteristics, mitochondrial activity, oil ratio (OR) index, survival and differentiation potential of mesenchymal stem cells of thawed adipose tissue were evaluated.

Results: The most damage or degeneration and OR indices of adipose tissues were detected in phosphate-buffered saline without any cryoprotectant at 1, 3, 6, and 9 months after cryopreservation (P≤0.05). The best protection of adipose tissue against freezing damage was observed when using a solution of 0.5 M DMSO + 9% FBS + 0.2 M trehalose (P < 0.05). Similarly, mitochondrial activities of thawed adipose tissues were the highest in the 0.5 M DMSO + 9% FBS + 0.2 M trehalose, but lowest in the phosphate-buffered saline. There was no difference in the stemness and differentiation potential of adipose tissue-derived mesenchymal stem cells among different cryopreservation treatments.

Conclusion: The combination of 0.5 M DMSO, 9% FBS and 0.2 M trehalose has the best protection for human adipose tissue during cryopreservation. Doi.org/10.54680/fr25310110412.

本刊更多论文

高效的人体脂肪组织冷冻保存程序，无需专门的冷冻设备。

背景：脂肪组织移植是修复损伤组织的重建方法之一。目的：建立一种无需特殊设备的人体脂肪组织冷冻保存方法。材料和方法：使用不同组合的渗透性和非渗透性冷冻保护剂冷冻脂肪组织，冷却速度为-1℃/ min，在-20℃下保存1、3、6和9个月。观察解冻脂肪组织间充质干细胞的组织形态学特征、线粒体活性、油比（OR）指数、存活和分化潜力。结果：在冷冻保存后1、3、6、9个月，不添加任何冷冻保护剂的磷酸盐缓冲盐水中脂肪组织的损伤或变性及or指数最高（P≤0.05）。0.5 M DMSO + 9% FBS + 0.2 M海藻糖对脂肪组织的冷冻损伤保护效果最好（P < 0.05）。同样，解冻脂肪组织的线粒体活性在0.5 M DMSO + 9% FBS + 0.2 M海藻糖中最高，而在磷酸盐缓冲盐水中最低。脂肪组织源性间充质干细胞的干性和分化潜能在不同低温保存处理间无差异。结论：0.5 M DMSO、9% FBS和0.2 M海藻糖联合使用对人体脂肪组织的低温保存保护效果最好。Doi.org/10.54680/fr25310110412。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.