Identification and expression analysis of cathepsin B genes in Myzus persicae (Hemiptera: Aphididae) and their response to environmental stresses.

IF 1.6 3区农林科学 Q2 ENTOMOLOGY

Bulletin of Entomological Research Pub Date : 2025-06-01 DOI:10.1017/S0007485325000136

Jin-Shan Zhang, Jian-Yu Meng, Lei Yang, Chang-Yu Zhang

{"title":"Identification and expression analysis of cathepsin B genes in Myzus persicae (Hemiptera: Aphididae) and their response to environmental stresses.","authors":"Jin-Shan Zhang, Jian-Yu Meng, Lei Yang, Chang-Yu Zhang","doi":"10.1017/S0007485325000136","DOIUrl":null,"url":null,"abstract":"Cathepsin B (CTSB) is a cysteine protease that is widely found in eukaryotes and plays a role in insect growth, development, digestion, metamorphosis, and immunity. In the present study, we examined the role of CTSB in response to environmental stresses in Myzus persicae Sulzer (Hemiptera: Aphididae). Six MpCTSB genes, namely MpCTSB-N, MpCTSB-16D1, MpCTSB-3098, MpCTSB-10270, MpCTSB-mp2, and MpCTSB-16, were identified and cloned from M. persicae. The putative proteins encoded by these genes contained three conserved active site residues, i.e. Cys, His, and Asn. A phylogenetic tree analysis revealed that the six MpCTSB proteins of M. persicae were highly homologous to other Hemipteran insects. Real-time polymerase chain reaction revealed that the MpCTSB genes were expressed at different stages of M. persicae and highly expressed in winged adults or first-instar nymphs. The expression of nearly all MpCTSB genes was significantly upregulated under different environmental stresses (38°C, 4°C, and ultraviolet-B). This study shows that MpCTSB plays an important role in the growth and development of M. persicae and its resistance to environmental stress.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"329-339"},"PeriodicalIF":1.6000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Entomological Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1017/S0007485325000136","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Cathepsin B (CTSB) is a cysteine protease that is widely found in eukaryotes and plays a role in insect growth, development, digestion, metamorphosis, and immunity. In the present study, we examined the role of CTSB in response to environmental stresses in Myzus persicae Sulzer (Hemiptera: Aphididae). Six MpCTSB genes, namely MpCTSB-N, MpCTSB-16D1, MpCTSB-3098, MpCTSB-10270, MpCTSB-mp2, and MpCTSB-16, were identified and cloned from M. persicae. The putative proteins encoded by these genes contained three conserved active site residues, i.e. Cys, His, and Asn. A phylogenetic tree analysis revealed that the six MpCTSB proteins of M. persicae were highly homologous to other Hemipteran insects. Real-time polymerase chain reaction revealed that the MpCTSB genes were expressed at different stages of M. persicae and highly expressed in winged adults or first-instar nymphs. The expression of nearly all MpCTSB genes was significantly upregulated under different environmental stresses (38°C, 4°C, and ultraviolet-B). This study shows that MpCTSB plays an important role in the growth and development of M. persicae and its resistance to environmental stress.

查看原文本刊更多论文

桃蚜（半翅目：蚜虫科）组织蛋白酶B基因的鉴定、表达及对环境胁迫的响应。

组织蛋白酶B （Cathepsin B， CTSB）是一种广泛存在于真核生物中的半胱氨酸蛋白酶，在昆虫生长、发育、消化、变态和免疫等过程中发挥重要作用。在本研究中，我们研究了CTSB在桃蚜（Myzus persicae Sulzer）响应环境胁迫中的作用。从桃科植物中鉴定并克隆了MpCTSB基因MpCTSB- n、MpCTSB- 16d1、MpCTSB-3098、MpCTSB-10270、MpCTSB-mp2和MpCTSB-16 6个基因。这些基因编码的蛋白含有三个保守的活性位点残基，即Cys、His和Asn。系统进化树分析显示，桃蚜的6个MpCTSB蛋白与其他半翼目昆虫高度同源。实时聚合酶链反应结果显示，MpCTSB基因在桃蚜不同阶段均有表达，在有翅成虫和一龄若虫中均有高表达。在不同的环境胁迫（38°C、4°C和紫外线b）下，几乎所有MpCTSB基因的表达都显著上调。该研究表明，MpCTSB在桃蚜的生长发育和对环境胁迫的抗性中起着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bulletin of Entomological Research 生物-昆虫学

CiteScore

4.00

自引率

0.00%

发文量

160

审稿时长

6-12 weeks

期刊介绍： Established in 1910, the internationally recognised Bulletin of Entomological Research aims to further global knowledge of entomology through the generalisation of research findings rather than providing more entomological exceptions. The Bulletin publishes high quality and original research papers, ''critiques'' and review articles concerning insects or other arthropods of economic importance in agriculture, forestry, stored products, biological control, medicine, animal health and natural resource management. The scope of papers addresses the biology, ecology, behaviour, physiology and systematics of individuals and populations, with a particular emphasis upon the major current and emerging pests of agriculture, horticulture and forestry, and vectors of human and animal diseases. This includes the interactions between species (plants, hosts for parasites, natural enemies and whole communities), novel methodological developments, including molecular biology, in an applied context. The Bulletin does not publish the results of pesticide testing or traditional taxonomic revisions.